Evaluation of Sbien Parameters in Ethiiopian Indigenous Bulls Kept at Kaliti, Artificial Insemination Center, Addis Ababa, Ethiopia
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Date
2004-06
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AAU
Abstract
The semen of six indigenous breeds bulls (Barca, Boran, Harro and Sheko) which were kept
al the N ationa! A rtificial l nsemination Center (NAlC). Kali li, Addis Ababa, Ethiopia were
evaluated for physico·morphological parameters (volume, color, mass and individual motility,
concentration, total count, percentage live and morphologically nonnal, total morphologically
nonnal and viable number), biochemical parameters (GOT, GPT, Aep, ALP, total protein)
and honnonai assay (testosterone). Semen was col lected for a thirteen week period from six
indigenous bulls kept at the Al center. The collection was done once per week using an
artificial vagina and semen sampll;:s were subjected to nomlai physico-morphological
characterization and seminal plasma was separated and preserved at -20 °C until analysis.
The overall mean (SE) value for t he indigenous bulls in the semen characteristics studied
were volume 4.84 (0.20) ml, mass motility 3.15 (0.11). individual motility 68.72 (1.37)
percent, concentration 1.54 (0.07) billion/ml, total count 7.35 (0.47) billion/ejaculate, viable
number 5.10 (0.33) billion/ejaculatl~, total morphologically nonnal number 7.12 (0.45)
billion/ejaculate, live percentage 79 .. 73 (0.65), percent morphologic nomlal 94.70 (0.38),
percent nomml acrosome 96.99 (0.60), GOT 1530.91 (60.15) UlL, GPT 131.99 (9.36) UIL,
ALP 3333 .98 (608.84) UIL, ACP 8003.68 (716.06) UIL, total protein 7.38 (0.26) gm/dL,
testosterone 2.84 (0.3) nglml, head abnonnality 1.87 (0.15) %, mid-piece (body) abnonnality
1.92 (0.22) %, tail abnormality 1.50 (0.18) %, total abnonnality 5.29 (0.38) %, major
abnonnality 2.25 (0.19) %, minor abnormality 3.04 (0.28) %, acrosome defect 3.0 (0.60) %,
narrow at base 0.37 (0.09) %, abnomlal contour 0.12 (0.04) %, undeveloped fonn 0.74 (0.15)
%, detached abnonnal 0.21 (0.09) %, pear shaped 0.05 (0.02) %, small abnonnal 0.05 (0.03)
%, abaxial attachment 0.57 (0.11) %, other head abnormality 0.07 (0.03) %, spennatozoa
head length 9.18 (0.03) "m, head breadth 4.61 (0.01) "m, mid-piece length 13.61 (0.02) "m,
tail length 47.56 (0.1) "m.
The semen physico-morphologic and seminal plasma biochemical parameters observed in this
study for the Ethiopian indigenous bulls are comparable to nomlai fertile bulls reported
elsewhere based on the evaluation of fresh semen parameters.
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Keywords
Ethiopia /NAIC/ lndigenous Breeds /Physico-Morphological Parameters/biochemical Parameters/Semen/Spennatozoa