Evaluation of Antihyperglycemic Effect of Moringa Stenopetala Aquoeous Leaves Extract on Alloxan Induced Diabetic Rats
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Date
2017-07
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Addis Abeba University
Abstract
Background: Diabetes is a serious metabolic disorder with complications that results in
significant morbidity and mortality. Current drugs used for diabetes therapy are not free
from side effects and do not restore normal glucose homeostasis. Therefore, the purpose of
this study is to evaluate antidiabetic, and pancreatic damage effect of Moringa stenopetala
aqueous extract on alloxan induced diabetic rats.
Methods:-The aqueous extract of Moringa stenopetala leaves 250 and 500mg/kg and
Glibenclimide 5mg/kg/ body weight were administered to alloxan induced diabetic rats.
All were administered orally using intragastric gavage for 28 days. Blood glucose level,
Histopathological examination, liver and kidney functions, preliminary phytochemical
screening tests were done.
Result:-After IP administration of Alloxan monohydrate, the fasted blood glucose level
rose in all experimentally induced diabetic groups. Administration of M. stenopetala leaves
aqueous extract to diabetic rats showed remarkably reduction in blood glucose
concentration, there were significant (P<0.05) difference on Day21 and 28 when compared
with diabetic group. Moreover, treatment with both doses of M. Stenopetala extract to
diabetic rats produced significant reduction in the blood glucose levels of rats when
compared with diabetic group. Continuous oral administration for 28 days of the plant for
diabetic rats led to a significant decrease in serum Urea, Creatinine, ALT, AST and ALP (P
< 0.05) as compared with diabetic group who showed significant concentrations. This
significant reduction was equivalent that occurred on application of glibenclamide. Levels
of serum cholesterol remained unaltered in the experimental groups when compared with
diabetic control. Histopathology of diabetic untreated rats revealed degeneration of
pancreatic islet cells, which was due to alloxan used in this experiment. However, signs of
regeneration of β-cells observed following consumption of Moringa stenopetala aqueous
leaves extract as reported.
Various phytochemical tests performed on the plant revealed the presence of different
secondary metabolites. M. stenopetala leaves aqueous extract (250 and 500mg/kg) were
improved the body weight of rats significantly from Day14-Day28 when compared with
the Diabetic group.
Conclusion:-The present study demonstrated that repeated oral administration of Moringa
stenopetala aqueous leaf extracts (250mg/kg and 500mg/kg) for 28 days has shown
beneficial effects on antihyperglycemia, body weight improvement restore biochemical
changes of blood, improve alloxan pancreatic damage. Moringa stenopetala aqueous
leaves extract have active ingredients responsible for antihyperglycemic effect. The
phytochemical screening indicated the presence of alkaloids, phenols, flavonoids, tannins,
terpenoids, steroids and saponins.
Keywords: Moringa stenopetala; Diabetes; Alloxan monohydrate
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Keywords
Moringa Stenopetala; Diabetes; Alloxan Monohydrate