Aflatoxin Status in Teff Produced By Commercial Farms for Export Purpose in Ethiopia
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Date
2019-06
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Addis Ababa University
Abstract
Conlalllinalion of cereal commodilies by mOlilds and lIIycoloxins resllils in cby IIwller,
qualily, and nlllrilional losses and represenls a signijicanl hazard 10 Ihe food chain
Ajlaloxins are loxic, hepalocarcinogenic, secondmy lIIeraboliles of Aspergillus species
prodllced in 1II0s1 agricullllral commodilies, slored 01 inappropriale lemperallires and lValer
aClivilies. Ajlaloxin conlaminalion of lef! in Erhiopia lVas reporled in 1987, indicaling Ihal
ill!;her probabililY of conI aminal ion of leff, sampled Fom differenl parI of Erhiopia.
The objeclive oflhis sludy lVas 10 invesligale occurrence of lola I ajlaloxin in lef!prodllced by
commercial farms of Elhiopia and Ihe ef!ecl of slored lemperallire and relalive humidilY lVas
also invesligaled In addilion, KAP sllldy lVas also conducled on OlVners and employees of
commercial farmers. A IOled of Eleven commercial lef! producing farms lVere randoml)!
selecled and, Ajlaloxin B1, B2, C I and C2 were delermined al microgram per kilogram
(JIg/kg) levels by immllno-ajjinily column clean lip and reversed-phase liquid
chromatography lVilh fluo rescence defection. The result showed all of Ihem were below the
maximllm (4 Ilg/kg) ,olerable level of Elhiopion lef! jlollr slandard The highesl AF
concenlralion lVas 3.99 JIg/kg and Ihe 10IVesl concenlralions lVas 0.5 JIg/kg. Blil 91%
cOnlaminaled by AFB1 and 45% lVere lInsaJe Jar direcl hllman consumplion as per Ihe
Elhiopian and EU maximllm (2 JIg/kg) ,olerable inlake level and, 27% oj Ihe samples velY
close (above 3pg/kg) 10 Ihe 4I'g/kg. 711e highesl ajlaloxin BllevellVasJound in lVesl Cojam
3.25 Ilg/kg and aj/aloxin B1 is nole delecled in sample form Adam Elhiopia. From Ihe
samples analysec/, 13. 88%, 0%, 8.38% and 77.73% were conlaminaled lV ilh qllanlifiable
concenlralions of ajlaloxin C2, C1, B2 and 81 respeclively. Ajlaloxin C2 (/3.88%) and BI
(77.73%) concenlralions lVere relalively higher Ihan Ihe olhers. In addilion, KA P sllldy lVas
condllcled on commercial farms and il sholVs Ihe/l, Ihe farmers had knolVledge on mycoloxin
re/a/ed question but their know/edge (0 AFs pracUce 100vards reducing the contaminalion
level of A Fs is nol enough.
Key word s: Ana toxin, Terr, KAP assessment, Immune-a affinity column
Description
Keywords
Anatoxin, Terr, KAP assessment, Immune-a affinity column