On Station Evaluation of Different Grains as Carrier for Thermostable (12) Newcastle Disease Vaccine Virus, Debrezeit,Ethlopia
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Date
2011-06-01
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Addis Ababa University
Abstract
In the current experimental study the suitability of different local grams as a carrrier for thermostable Newcastle disease vaccine 12 for oral immunization of chickens was assessed by haemagglutination inhibition test and challenge experiment. The study was conducted at school of veterinary medicine, Debrezeit, Ethiopia from December 20 10 until June 20 II. A total of 225 chicks were divided in to 15 treatment groups and each treatment group had 15 chickens kept in separate pens. The chickens were vaccinated with 12 vaccine by conventional methods (drinking water), cracked maize and wheat, millet, barely and sorghum which were used in untreated , parboiled and cracked form. Chickens were vaccinated three times at the age of 2, 5, 15 weeks Following the primary and the second vaccination the result showed that only chickens vaccinated with conventional methods and vaccine coated cracked maize and parboiled barely gave GM antibody titer of 2: log23 but at third dose of vaccine except for chickens vaccinated with untreated and cracked form of sorghum, barely, millet all vaccinated birds showed GM antibody titer of 2: log2232. At this time chicken vaccinated by conventional, cracked maize and parboiled barely were found to produce the highest GM titer of log227.1, log226.s and log225.1 respectively but the least response (log22 I 9) was observed in chicken vaccinated by vaccine coated cracked sorghum. The experimental challenge showed that 80% mortality rate in the unvaccinated control group. The vaccine applied by drinking-water, cracked maize, parboiled barely was found 100% protective against the challenge. High protection capacity was also observed with 12 delivered by parboiled wheat (85%). Similarly 80% protection rate was found with vaccine coated untreated wheat, and parboiled sorghum. Limited survival rate ( 60%) was observed in the chicken under, untreated millet and cracked millet treatment group. The least survival rate with high mortality was observed in chicken treated with vaccine coated sorghum. Generally in the current experimental study cracked maize, parboiled barley, parboiled wheat, parboiled sorghum and untreated wheat found to be good candidate carrier for 12 vaccine virus
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Keywords
Grains, Challenge experiment, Chickens, Debreziet, Ethiopia haemagglutination inhibition, Newcastle diesese, Survival rate