Bacterial profiles and antibacterial susceptibility patterns of frequently hand touched surfaces on public transport buses, Automated Teller Machines and St’ Paul Hospital patient waiting areas in Addis Ababa, Ethiopia
No Thumbnail Available
Date
2019-06
Authors
Akele, Mamo
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababa University
Abstract
Background: Frequently touched surfaces on Automated Teller Machines, in public transport buses and hospital environment can constitute reservoir for healthcare and community associated infections and may represent a mechanism for the spread of these infection in the community.
Objective: To assess bacterial profiles and antibacterial susceptibility patterns of hand touched surfaces in public transport buses, Automated Teller Machines and Hospital waiting areas inanimate object in Addis Ababa city.
Methods: A cross sectional study was conducted from Jan 2018 to Sep 2018. A total of 423 samples were collected from frequent hand touch surfaces of selected Automated Teller Machine, public transport buses and hospital waiting area inanimate objects located in Addis Ababa, Ethiopia by using sterile cotton swaps. The bacterial isolates were examined and identified by colonial morphology, Gram reaction and biochemical characteristics. Antibiotic susceptibility test was done by disc agar diffusion method. Data analysis was done by using SPSS version 23
Results: Growth was seen in all samples. From the total (n=588) bacterial isolates, 75.7% and 24.3% were Gram-positive and Gram-negative, respectively. Coagulase negative Staphylococcus species, Staphylococcus aureus, Proteus species, Klebsiella species, Pseudomonas species, Acinetobacter species and Enterobacter species were commonly isolated bacteria. It is very dreadful to observe that and some of these bacteria are highly resistant to the commonly used antibiotics such as, 71.3%, 67.3% and 40.2% of Staphylococcus species showed resistant against to tetracycline, penicillin and oxacillin, respectively. Proteus Spp was resistant to cefuroxime 77.6%, ampicillin 79.3%, ceftriaxone 84.5% and ceftazidime 84.5%. Moreover, in 72.0% of the total isolate multidrug resistance was observed.
Conclusion: These bacteria identified have pathogenic potential and hence their presence on those object surfaces may have a capability of transmitting pathogens through the community is an indicative of the need for awareness on cleaning of such surfaces or disinfection and adequate hand hygiene.
Description
Keywords
Automated Teller Machine, Public transport bus, Hospital inanimate object, Antimicrobial resistant, Bacterial profiles, Ethiopia.