Evaluation of Trichoderma Species as Biological Control Against Fusarium Wilt (Fusarium Spp.) on Cotton Plant (Gossypium Herbaceum) Under in Vitro Condition
| dc.contributor.advisor | Alemu, Tesfaye (PhD) | |
| dc.contributor.author | Asmekash, Tsegay | |
| dc.date.accessioned | 2020-11-19T12:35:55Z | |
| dc.date.accessioned | 2023-11-09T04:20:52Z | |
| dc.date.available | 2020-11-19T12:35:55Z | |
| dc.date.available | 2023-11-09T04:20:52Z | |
| dc.date.issued | 2019-10-10 | |
| dc.description.abstract | In Ethiopia, cotton is one of the most important cash crops that widely grown in the country. The production trend of cotton has been on the decline over the years, mostly as a result of infectious diseases from fungal pathogens. Thus the overall objective of the study was to evaluate local Trichoderma isolates for their pathogenicity potential against Fusarium isolates. The ability of the isolates to antagonize Fusarium isolates was evaluated in invitrodual culture assays and using volatile and non-volatile metabolites produced by the isolates.The effect of temperature and pH on the growth of Trichodermaisolates was also evaluated under a pH value ranging from 4.5-7.5 and level of temperature at 4oC, 25oC and 37oC.The inhibition test effects were analyzed using the analysis of variance (ANOVA) and the significance of the means was evaluated by Tukey‟s HSDtests. Out of27 isolates one isolate wasmorphologicaly identified as Fusarium pathogen isolate then selected and subjected to pathogenicity and antagonize test effects.The pathogenicity result showed the typical symptoms of the pathogen on the inoculated detached leaves and seedlings.Among the fourTrichodermaisolates, T131 showed the highest inhibitory 78.89% effect against the pathogen at based on dual culture assay. Use of volatile and non-volatile metabolites produced by the Trichoderma isolates also confirmed that there was a production of inhibitory substances.TheTrichoderma isolatesT131 (48.14%) and T136 (52.89%) showed the highest inhibitory effect against the pathogen.It is clearly observed fro the results of Trichodermaisolates were successfully grown at 25oC and 37oC.Similarly,Trichodermaisolates sporulated at different pH values. However, the pH change was not significant except in Trichoderma isolate GimT7. In the present study, T 131 were found to be the best isolate against cotton wilt pathogen (Fusarium spp). | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/23399 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Antagonistic Activity | en_US |
| dc.subject | Secondary Metabolites | en_US |
| dc.subject | Dual Culture | en_US |
| dc.subject | Pathogenicity | en_US |
| dc.title | Evaluation of Trichoderma Species as Biological Control Against Fusarium Wilt (Fusarium Spp.) on Cotton Plant (Gossypium Herbaceum) Under in Vitro Condition | en_US |
| dc.type | Thesis | en_US |