PATHOLOGICAL CHARACTERIZATION OF LESIONS AND BACTERIOLOGICAL ISOLATION OF CAUSATIVE AGENTS OF SWINE TUBERCULOSIS AT BISHOFTU AND ADDIS ABABA ABATTOIRS, BISHOFTU, ETHIOPIA
| dc.contributor.advisor | Dr. Tilaye Demissie, Gobena Ameni (Prof) | |
| dc.contributor.author | JIRATA, SHIFERAW | |
| dc.contributor.author | Dr. Gezahegne Mamo, Dr. Kasa Demissie | |
| dc.date.accessioned | 2018-09-28T08:51:08Z | |
| dc.date.accessioned | 2023-11-30T13:38:36Z | |
| dc.date.available | 2018-09-28T08:51:08Z | |
| dc.date.available | 2023-11-30T13:38:36Z | |
| dc.date.issued | 2018-06 | |
| dc.description | A Thesis Submitted to the College of Veterinary Medicine of Addis Ababa University in Partial Fulfillment of the Requirements for the Degree of Master of Veterinary Science in Veterinary Pathology | en_US |
| dc.description.abstract | A cross sectional study was conducted at Bishoftu and Addis Ababa Abattoirs from September 2017 to May 2018 to estimate abattoir based prevalence of tuberculosis in swine, to isolate Mycobacterium species involved and to characterize the lesions. Five hundred and fifty six (556) swine were examined. Tubercle like granulomatous lesion were detected in 19.6% (109/556) of different organs of which 12% (69/556) was in lymph nodes, 5.7% (32/556) in the lungs, 1% (6/556) in the liver and 0.36% (2/556) in the spleen. Based on microscopic changes 4.7% (26/556) of lesions were tuberculous granulomatous type with central necrosis, calcified foci, epitheloid cells admixed with lymphocyte and connective tissue boundries, 3.6% (20/556) pyogranulomatous and 2.5% (14/556) non necrotic granulomatous lesion without epitheloid cells and connective tissue capsules. In lymph nodes, severe depletion of lymphocytes, calcification, cellular debris and aggregation of macrophages were observed microscopically. The tuberculous lesions found in lymph node were statistically significant (P < 0.05) than in other organs. The multivariable logistic regression analysis showed that old aged swine were more likely to have characteristic tuberculous lesion (OR = 3.14, 95% CI, 1.62-6.09) than younger ones. From the tissue cultured, 7.5% (3/40) yield growth on primary culture media. The observed colony morphology was smooth whitish or yellowish color, sticky, off-white and breaks apart easily and two (5% (2/40)) of these growth were acid fast positive by Zeihl-Neelsen staining technique. Based on culture result, and typical histopathologic lesions it could be concluded that pork can be source of tuberculosis to human when consumed under cooked and routine abattoir inspections should be conducted. Therefore, further molecular and biochemical research to isolate the species of microbacteria is highly recommended to elucidate the type of lesion and its magnitude in different body organs by considering large number of swine. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/12304 | |
| dc.language.iso | en | en_US |
| dc.subject | Abattoir | en_US |
| dc.subject | Lesion | en_US |
| dc.subject | Zeihl-Neelsen | en_US |
| dc.title | PATHOLOGICAL CHARACTERIZATION OF LESIONS AND BACTERIOLOGICAL ISOLATION OF CAUSATIVE AGENTS OF SWINE TUBERCULOSIS AT BISHOFTU AND ADDIS ABABA ABATTOIRS, BISHOFTU, ETHIOPIA | en_US |
| dc.type | Thesis | en_US |