Investigation of Strain Diversity and Trend of Drug Resistance Pattern of the Clinical Isolates from Tuberculosis Lymphadenitis Patients in Two Different Cities of Ethiopia
| dc.contributor.advisor | Desta Kassu | |
| dc.contributor.advisor | Hailu Melese | |
| dc.contributor.advisor | Araya hambel | |
| dc.contributor.author | Sebri Sami | |
| dc.date.accessioned | 2024-11-28T09:53:30Z | |
| dc.date.available | 2024-11-28T09:53:30Z | |
| dc.date.issued | 2023-06 | |
| dc.description.abstract | Background: Identification of Mycobacterium tuberculosis strain have a significant contributionto the understanding of genetic diversity in a particular area. The most commonly occurring typeof extra-pulmonary tuberculosis is TB Lymphadenitis. Tuberculosis (TB) is a complicated andchronic infectious disease and severely affect the organs. Investigating and understanding theassociation between Molecular characteristics and drug susceptibility of (MTBC) strains areimportant to comprehend the transmission dynamics and drug resistance pattern. Objective: To investigate strain diversity and trends of drug resistance pattern of the clinicalisolates from Tuberculosis lymphadenitis (TBLN) patients in two different cities of Ethiopia. Methods: Institution-based cross-sectional study was conducted from 52 previously stored isolatescollected from TBLN patients in 2019 from Ambo and from ALERT comprehensive hospital in2021. Sub-culture using LJ, Line Probe Assay, and Spoligotyping wereperformed from the storedisolates. Descriptive statistics were used to present frequency of data. A statistical analysis wasdone using SPSS Version 26. A test with a P value of < 0.05 was considered statisticallysignificant. Results: A total of 52 culture positive confirmed M. tuberculosis isolates have been used for drugsensitivity testing and strain identification. Out of the 52 spoligotype patterns (strains), 29 isolatesmatched the preexisting patterns in the SITVIT2 database, the rest 23 found to be new or orphanstrains according to SITVIT2 database. Classification of MTBC strains from both cities showedthe occurrence of the following predominant lineages, Euro-American (40/52 76.9%), followed byIndo-Oceanic (2/52; 3.8%), and East-African (9/52;17.30%). isolates in two different cities wereinvestigated, isolates from ambo collected in 2019 identified to be sensitive for all major anti-TBdrugs. However, culture positive samples collected in 2021 from Addis Ababa have shownresistant strain for both anti TB-drugs, 4 (15%), 3(11.5%) of them have been resistance to bothRIF and INH, and the rest 1 isolate were Mono- resistant to INH 1 (3.8%). Conclusion: This study determines the association of drug resistant TB with corresponding lineageand molecular epidemiology in patients with tuberculous lymphadenitis. Lineage 3 and Lineage 4represented to be most prevalent lineage of M. tuberculosis with high clustering rates of SIT 125,SIT149 and SIT 53, more over these lineages are associated with most of drug resistance M.tuberculosis. | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/3747 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Ababa University | |
| dc.subject | Extra-Pulmonary tuberculosis Line probe assay and Drug resistance. | |
| dc.title | Investigation of Strain Diversity and Trend of Drug Resistance Pattern of the Clinical Isolates from Tuberculosis Lymphadenitis Patients in Two Different Cities of Ethiopia | |
| dc.type | Thesis |