Prevalence of Intestinal parasites, Salmonella and Shigella, Associated Risk Factors and Antibiotics Susceptibility Pattern of isolates amongfood handlers in Addis Ababa police commission camps, Addis Ababa, Ethiopia.
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Date
2021-10
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Addis Ababa University
Abstract
Background:Diseases such as Salmonellosis,Shigella, and intestinal parasites remain a major
public health problem worldwide. The problem is especially acute in developing countries due to
the personal hygiene and handling practices of food processors. Food handlers have been caught
by various pathogens and are being transmitted to the police. The purpose of this study was to
examine the presence of intestinal parasites, Salmonella and Shigella, associated risk factors and
ant ibio t ics susceptibility pattern of iso lates among food handlers in Addis Ababa police
commission camps, Addis Ababa, Ethiopia.
Methods:From February to May 2021, cross-section study were conducted in ten sub-cit ies
selected by Addis Ababa Police Commission Camps. Social demographic data and fecal samples
were collected from 247 study participants. Stool specimens were tested by wet mount and
float at ion, and sedimentat ion with microscopic.The stoolculturewasdone onXylose Lysine
Deoxycholate agar (XLD) and Deoxycholate citrate agar for(DCA) SalmonellaandShigella
ident ified on the biochemical tests(nutrient broth,KIA, Citrate, LIA, Urea, and Mot ilit y) and also
Salmonella agglutinating test performed.Ant imicrobial suscept ibilit y test was doneon Muller
Hinton agar plates against ampicillin (10 μg), ceftriaxone (30 μg), chloramphenicol (30 μg), and
ciprofloxacin (5 μg). The Data were entered, coded and analyzedwith SPSS version 25.
The
associations between risk factor and intestinal parasite were tested using the Chi-square test. P
values ≤ 0.05 were considered to indicate statistical Significance.
Results:The majority of food handlers (83%) were young people aged less than 26,the mean age
of the respondents 22.7, standard division 5, and range 38. All the food handlers were women.
One hundred and forty-three (57.9%) food handlers were educated beyond pr imary school. Most
(54.3%) of food handlers were serve below one years. A 98.8% of the participants interviewed
had a toilet in the workplaceand wash their hands before serving and serving any food. Similarly,
most the respondents 179 (72.5%) said that when they suffer from diseases such as diarrhea, they
do not prepare food.Twenty-nine (11.74%) of the participants had intestinal parasites.
Entamoebahistolytica/dipar was the most widespread parasite (5.7%) of those who provided
stool samples. In addition, 1.6% (4/247) of the samples was positive for Salmonella. All o f the
Salmonella isolates were sensitive for gentamicin, ciprofloxacin, ceftriaxone, tetracycline,
chloramphenicol, amoxicillin / clavulanic acid, ampicillin-sulbactam, Nacidixcacid,
cotrimoxazole, imipenem, and marocain and all isolates were resistant to antibiotics like
Ampicillin, Ceftazidime, Cefotaxime, and Cefuroxime.
Conclusions:
We conclude that the health problems in the study area areintest inal parasite and Salmonella. To
address foodborne illness in Addis Ababa Police Commission Camps regular health education
and training programs among food handlers had requested to address.
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Keywords
Intestinal parasite, Salmonella, Shigella, food handler