Prevalence of Intestinal Parasites, Salmonella and Shigella, Associated Risk Factors and Antibiotics Susceptibility Pattern of isolates among Food Handlers in Addis Ababa Police Commission Camps, Addis Ababa, Ethiopia.
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Date
2021-10
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Addis Ababa University
Abstract
Background:Diseases such as Salmonellosis,Shigella, and intestinal parasites remain a majorpublic health problem worldwide. The problem is especially acute in developing countries due tothe personal hygiene and handling practices of food processors. Food handlers have been caughtby various pathogens and are being transmitted to the police. The purpose of this study was toexamine the presence of intestinal parasites, Salmonella and Shigella, associated risk factors andant ibio t ics susceptibility pattern of iso lates among food handlers in Addis Ababa policecommission camps, Addis Ababa, Ethiopia.
Methods:From February to May 2021, cross-section study were conducted in ten sub-cit iesselected by Addis Ababa Police Commission Camps. Social demographic data and fecal sampleswere collected from 247 study participants. Stool specimens were tested by wet mount andfloat at ion, and sedimentat ion with microscopic.The stoolculturewasdone onXylose LysineDeoxycholate agar (XLD) and Deoxycholate citrate agar for(DCA) SalmonellaandShigellaident ified on the biochemical tests(nutrient broth,KIA, Citrate, LIA, Urea, and Mot ilit y) and alsoSalmonella agglutinating test performed.Ant imicrobial suscept ibilit y test was doneon MullerHinton agar plates against ampicillin (10 μg), ceftriaxone (30 μg), chloramphenicol (30 μg), andciprofloxacin (5 μg). The Data were entered, coded and analyzedwith SPSS version 25.Theassociations between risk factor and intestinal parasite were tested using the Chi-square test. Pvalues ≤ 0.05 were considered to indicate statistical Significance.
Results:The majority of food handlers (83%) were young people aged less than 26,the mean ageof the respondents 22.7, standard division 5, and range 38. All the food handlers were women.One hundred and forty-three (57.9%) food handlers were educated beyond pr imary school. Most(54.3%) of food handlers were serve below one years. A 98.8% of the participants interviewedhad a toilet in the workplaceand wash their hands before serving and serving any food. Similarly,most the respondents 179 (72.5%) said that when they suffer from diseases such as diarrhea, theydo not prepare food.Twenty-nine (11.74%) of the participants had intestinal parasites.Entamoebahistolytica/dipar was the most widespread parasite (5.7%) of those who providedstool samples. In addition, 1.6% (4/247) of the samples was positive for Salmonella. All o f theSalmonella isolates were sensitive for gentamicin, ciprofloxacin, ceftriaxone, tetracycline, chloramphenicol, amoxicillin / clavulanic acid, ampicillin-sulbactam, Nacidixcacid, cotrimoxazole, imipenem, and marocain and all isolates were resistant to antibiotics likeAmpicillin, Ceftazidime, Cefotaxime, and Cefuroxime.
Conclusions:We conclude that the health problems in the study area areintest inal parasite and Salmonella. Toaddress foodborne illness in Addis Ababa Police Commission Camps regular health educationand training programs among food handlers had requested to address.
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Keywords
Intestinal parasite, Salmonella, Shigella, food handler