The Toxic Effects of Vernonia Bipontini on Some Blood Parameters and on Liver and Kidney Tissues
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Date
2009-05
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Addis Abeba University
Abstract
Toxicological studies are sources of useful information for evaluating the therapeutic
benefits of locally used medicinal plants. Vernonia bipontini (V. bipontini) is a herb used
for treatment of malaria and malaria related symptoms in Ethiopia. However, its side
effects have not been studied. In this study, the toxic effects of aqueous and methanolic
extracts of V. bipontini leaves on some blood parameters and on liver and kidney, tissues
in mice were investigated.
Lethal doses at which 50% of experimental mice died (LD50s) in both aqueous and
methanolic leaf extracts of V. bipontini were determined by administering different doses
(1250-3250mg/kg for aqueous leaf extract and 1250-2750mg/kg for methanolic leaf
extract) to experimental animals using intragastric catheter. For long-term toxicity study,
sixty male and female Swiss albino mice were equally divided into six groups of 10
animals each. Groups1 and 2 were set as the control and received 0.5ml of distilled water
and 0.5ml of 4% tween in distilled water, respectively, at 24 hrs intervals for 45 days.
Group 3 and 4 were subjected to oral administration of the aqueous leaf extract at 400
and 800mg/kg, respectively, while group 5 and 6 were treated at 400 and 800mg/kg of
methanolic leaf extract, respectively in 24 hrs intervals for 45 days. All groups were
closely observed for any physical and behavioral alterations. Body weights of the mice
were recorded on the first day and the last day of administration. Each animal was
sacrified under diethyl ether anesthesia on the 46th day. Following sacrifice, blood sample
was collected by cardiac puncture using sterile needle and 5ml syringe into heparinized
test tubes for hematological studies and into non-heparinized tubes for biochemical
analysis. Hematological parameters (total RBC, WBC, platelets, lymphocytes, Hgb, Hct,
Mcv, Mch, and Mchc) and biochemical parameters (AST, ALT, ALP, and urea) were
evaluated. Through a vertical, midline incision, the liver and kidney of each animal were
removed and cleaned of the surrounding tissues. Each sample was fixed in 10% buffered
formalin overnight. The tissues were processed for light microscopy.
The LD50s of the aqueous and methanolic leaf extracts of V. bipontini were 2500.62±5.24
mg/kg and 2130.6±1.5mg/kg, respectively. No deaths were recorded among the control
groups. The aqueous leaf extract has no significant (P>0.05) effect on liver and kidney
weights, and hematological and biochemical parameters at all doses when compared with
control group. Treatment with 800mg/kg body weight of methanolic leaf extract
significantly (P<0.05) decreased body, liver and kidney weights, RBC, Hgb, Mch, Mchc,
platelets and significantly increased serum AST, ALT and ALP levels while 400mg/kg
dose had no effect on these parameters. The reduced organ weights did not correlate with
loss of body weight at 800mg/kg bw of methanolic leaf extract of the plant.
Light microscope observations of liver tissue of mice treated with 800mg/kg of the
methanolic leaf extract revealed dilated sinusoids, nuclear enlargement, bi-nucleation of
hepatocytes, peripheral cramped chromatin, shrinkages (single cell death) of hepatocytes,
fragmentation of hepatocytes (apoptosis) while no histopathological changes were
observed in liver and kidney of mice treated at 400mg/kg of the methanolic leaf extract
and at all doses of aqueous leaf extract. Kidney tissue sections of mice did not show
significant histopathological changes at 400mg/kg of the same methanolic leaf extract of
V. bipontini. However, at 800mg/kg kidney sections showed that increase cellularity of
glomerulus and urinary space obliteration.
In conclusion, this study suggests that the aqueous leaf extract of this plant may be safe,
even when taken for 45 days at higher dose (800mg/kg). This is in agreement with
traditional claim of the water preparation of V. bipontini leaves. However, methanolic
leaf extract may be phytotoxic to liver that resulted in a rise in serum AST, ALT and ALP
levels after 45 days herbal administration at high dose. Further studies would be needed
to identify the active ingredients responsible for such toxicities.
Key words: V. bipontini, Swiss albino mice, Liver, Kidney, Hematological and
Biochemical parameters
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Keywords
V. Bipontini, Swiss Albino Mice, Liver, Kidney, Hematological and Biochemical Parameters