Evaluation of Antidiarrheal, Antimicrobial, and Antioxidant Activities of 80% Hydro-Methanolic Root and Leaf Extracts of Carissa Spinarum
| dc.contributor.advisor | Lishan Asefa | |
| dc.contributor.author | Berhanu Tafese | |
| dc.date.accessioned | 2024-11-04T11:34:35Z | |
| dc.date.available | 2024-11-04T11:34:35Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Carissa spinarum L is a medicinal plant with therapeutic and nutritional properties; however, scientific evidence of its antidiarrheal and antibacterial potential against resistant bacteria is lacking. Similarly, there is limited evidence available about its anti-oxidant properties, particularly on the root and leaf. Thus, the current study aimed to evaluate the antidiarrheal and antibacterial activities and antioxidant capacities of 80% methanolic root and leaf extracts of Carissa spinarum. Each root and leaf powder was extracted by maceration technique using 80% methanol as a solvent. The effects of the crude extract on entero-pooling, gastrointestinal motility tests, and castor oil-induced diarrhea were investigated at 100, 200, and 400 mg/kg doses. The antibacterial activity was conducted by the broth dilution method, and the antioxidant activity was carried out by the 2-2-Diphenyl-1-picrylhydrazyl method. The root and leaf extracts prolonged the onset of diarrhea and reduced the number of wet feces dropped and the total weight of feces significantly compared to the negative control at doses of 200 and 400 mg/kg (p<0.001). The results of the enteropooling test demonstrated that the extracts at 200 and 400 mg/kg (p<0.001) significantly decreased the weight and volume of the intestinal content. At the test dose of 400 mg/kg, both the leaf and root extracts significantly (p<0.001) decreased the weight and volume of intestine content in the enteropooling experiment compared to the negative control group, as well as the 100 and 200 mg/kg doses. The root and leaf extracts at all test doses significantly reduced intestinal motility compared to the negative control (p<0.001). In the antibacterial activity test, it was shown that both root and leaf extracts exhibited comparable activity against resistant and susceptible bacteria but showed superior efficacy against resistant strains. The antioxidant assay demonstrated that both plant extracts exhibited promising activity and were comparable to the standard with their inhibitory concentrations (IC50) of (12.61±0.51), (13.6±0.28), and (5.86±0.35µg/ml) of root, leaf, and ascorbic acid, respectively. Thus, the methanolic root and leaf extracts of Carissa spinarum support the traditional claims of antidiarrheal, antibacterial, and antioxidant properties. However, further research is needed to elucidate the mechanisms behind antibacterial and antidiarrheal activity, and there is also a need to validate the antioxidant capacity through in vivo assays | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/3534 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Abeba University | |
| dc.subject | Antibacterial | |
| dc.subject | Antidiarrheal | |
| dc.subject | Antioxidant | |
| dc.subject | Carissa spinarum | |
| dc.subject | Extract | |
| dc.title | Evaluation of Antidiarrheal, Antimicrobial, and Antioxidant Activities of 80% Hydro-Methanolic Root and Leaf Extracts of Carissa Spinarum | |
| dc.type | Thesis |