Staphylococcus Aureus and MRSA Contamination Level of Working Clothe, Hand Carriage, and Knowledge, Attitude and Practice Among Healthcare Workers at Federal Police Hospital; Addis Ababa, Ethiopia.
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Date
2021-11
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Addis Abeba University
Abstract
Background: Contaminated workings clothe and hand of health care workers can cause
nosocomial infections. Staphylococcus aureus (S aureus) is the main causative agent of
nosocomial infection and it can be transmitted through working clothe of health care workers
from patient to health care workers and from health care workers to patients at hospitals. Poor
hand hygiene is the main way of spreading infectious organisms.
Objective: To assess Staphylococcus aureus and MRSA contamination level of Working Clothes,
Hand carriage, and knowledge, attitude, and practice (KAP) among Health Care workers
(HCWs) at Federal Police Hospital.
Methods: Cross-sectional study was conducted at the Federal police hospital (FPH) from
November 2020-March 2021 by inclusion of 222 healthcare workers. 222 swab samples were
collected from the working clothing of all participants and 53 hand swab samples were randomly
collected from 53 HCWs. Culturing, identification, and antimicrobial sensitivity tests were
performed at the Federal Police Hospital microbiology laboratory using conventional standard
microbiology tests. The positive and negative control organisms' S aureus and S epidermidis
were obtained from Ethiopian Public Health Institute (EPHI) microbiology department. The
demographic data, knowledge, attitude, and practice of study participants were collected by
providing self-administered questioner and observational checklists. The data entry was done by
Microsoft Excel and chi-square data analysis done by using SPSS version 21 software.
Results: Out of 222 study participants, 39(17.6%) were contaminated with staphylococcus
bacteria. A total of 41 staphylococci were isolated from 35 working clothes,2 hands, and 2 were
isolated from both clothes and hands. From a total of 41 isolates, 18(43.9 %) S aureus, and
23(56.1%) coagulase-negative Staphylococcus (CoNS) were identified. 12(67%) Methicillin
sensitive S aureus (MSSA) and 6(33%) Methicillin-resistant S aureus (MRSA) were identified
from a total of 18 S aureus isolates. About 86.9% of study participants have good knowledge
about the use and handling of working clothes related to bacterial contamination. 60% of them
show a good attitude and 70.1% were practicing good on using and handling of working clothes
related to the prevention of bacterial contamination.
Conclusion: In this study the contamination rate of working clothe and hand carriage of S
aoreus was 6.8% and 5.6% respectively and 33% of the isolates were MRSA. Even though study
participants have good knowledge, attitude, and practice about working clothe and hand hygiene
continuous awerness of staff is paramount to prevent MRSA in the hospital.
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Keywords
Clothe contamination, S aureus , MRSA , Healthcare worker, hand-carriage, Nosocomial.