Micropropagation of Violet tree (Securidaca Longepedunculata, Fresen.), an Endangered Ethiopian Medicinal Plant from Shoot Tip
| dc.contributor.advisor | Feyissa Tileye | |
| dc.contributor.author | Lijalem Teklebrihan | |
| dc.date.accessioned | 2018-06-18T07:12:57Z | |
| dc.date.accessioned | 2023-11-18T09:50:12Z | |
| dc.date.available | 2018-06-18T07:12:57Z | |
| dc.date.available | 2023-11-18T09:50:12Z | |
| dc.date.issued | 2014-07 | |
| dc.description.abstract | Securidaca longipedunculata Fresen, locally called Etse-manahi, is semi-deciduous shrub or small tree that belonging to the family Polygalaceae and found in rainfall andequatorial humid areas of Africa. It is an indigenous medicinal plant in Africa and hasimportant place in the traditional medicine as well as modern medicine. This plantbecomes endangered because of high seed dormancy, low germination rate, overexploitation and long growth period. Therefore, micropropagation method is importantto address these problems. Thus, the objective of this study is to developmicropropagation protocol for S. longipedunculata from shoot tip explants. Seeds were de-coated and disinfected using 3%, 5%, 10% and 15% (v/v) Clorox and cultured on growth regulators-free solid MS medium. Shoot tips were initiated using variousconcentrations of 6-Benzyl Amino Purine (BAP) in combination with Thidiazuron. MSmedium containing different concentrations of BAP in combination with IBA; TDZ incombination with IBA or NAA were used for shoot multiplication. Shoots were culturedon full and half strength MS medium containing IAA, IBA or NAA for root induction. Thehighest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0mg/l BAP. Maximum number of shoots (8.5 ± 0.69) was achieved on MS multiplicationmedium containing 1.5 mg/l BAP in combination with 0.1 mg/l IBA. Shoots gave highestnumber of roots (3.73 ± 0.69) on full strength MS rooting medium containing 2.0 mg/lIAA. Among 102 plantlets transferred to greenhouse, 61 plantlets survived on the potcontaining soil, sand and compost in a ratio of 2:1:1 respectively. Key words/phrases: ex vitro; in vitro; Micropropagation; plant growth regulator,Securidaca longipedunculata, shoot initiation; shoot tip culture | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/1147 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | ex vitro; in vitro; Micropropagation; plant growth regulator, Securidaca longipedunculata, shoot initiation; shoot tip culture | en_US |
| dc.title | Micropropagation of Violet tree (Securidaca Longepedunculata, Fresen.), an Endangered Ethiopian Medicinal Plant from Shoot Tip | en_US |
| dc.type | Thesis | en_US |