Micropropagation of violet tree (Securidaca longepedunculata, Fresen.), an Endangered Ethiopian medicinal plant from Shoot tip
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Date
2014-07
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Addis Ababa University
Abstract
Securidaca longipedunculata Fresen, locally called Etse-manahi, is semi-deciduous shrub or small tree that belonging to the family Polygalaceae and found in rainfall and
equatorial humid areas of Africa. It is an indigenous medicinal plant in Africa and has
important place in the traditional medicine as well as modern medicine. This plant
becomes endangered because of high seed dormancy, low germination rate, over
exploitation and long growth period. Therefore, micropropagation method is important
to address these problems. Thus, the objective of this study is to develop
micropropagation protocol for S. longipedunculata from shoot tip explants. Seeds were de-coated and disinfected using 3%, 5%, 10% and 15% (v/v) Clorox and cultured on growth regulators-free solid MS medium. Shoot tips were initiated using various
concentrations of 6-Benzyl Amino Purine (BAP) in combination with Thidiazuron. MS
medium containing different concentrations of BAP in combination with IBA; TDZ in
combination with IBA or NAA were used for shoot multiplication. Shoots were cultured
on full and half strength MS medium containing IAA, IBA or NAA for root induction. The
highest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0
mg/l BAP. Maximum number of shoots (8.5 ± 0.69) was achieved on MS multiplication
medium containing 1.5 mg/l BAP in combination with 0.1 mg/l IBA. Shoots gave highest
number of roots (3.73 ± 0.69) on full strength MS rooting medium containing 2.0 mg/l
IAA. Among 102 plantlets transferred to greenhouse, 61 plantlets survived on the pot
containing soil, sand and compost in a ratio of 2:1:1 respectively.
Key words/phrases: ex vitro; in vitro; Micropropagation; plant growth regulator,
Securidaca longipedunculata, shoot initiation; shoot tip culture
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Keywords
ex vitro; in vitro; Micropropagation; plant growth regulator, Securidaca longipedunculata, shoot initiation; shoot tip culture