Analysis of Genetic Diversity Among Cultivated Enset (Ensete Ventricosum) Populations from Essera and Kefficho, Southwestern Part of Ethiopia Using ISSR Marker
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Date
2008-06
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Addis Ababa University
Abstract
This study was conducted with the objective of investigation of genetic
variation between populations of cultivated enset and provides information for
conservation and improvement of enset. Leaf samples for seventy-one enset
plants were collected from two areas of SW Ethiopia (Kefficho and Essera)
and genomic DNA was extracted from the samples CTAB extraction method.
Inter simple sequence repeats (ISSRs) as a molecular marker was used to
assess genetic diversity both within and between populations using two ISSR
primers (834 and 826). A total of 26 clear and reproducible bands were
amplified from the two primers. Both UPGMA and neighbor joining trees were
constructed for each individual and population using Jaccard’s similarity
coefficient. The trees indicated two separate clusters which are based on the
origin of populations and few intermixing of samples between regions was
observed. The PCO analysis also recovered the UPGMA and neighbor joining
trees groups. All parameters indicated that the clones of enset from Kefficho
are more diverse than clones from Essera. Moreover, partioning of Shannon’s
diversity index showed that the majority of the variations were observed within
populations of enset than between the two populations. In conclusion, the
present study using ISSR analysis, all the diversity parameters confirm that
there is high diversity in cultivated Ensete ventricosum populations of SW
Ethiopia and that ISSR markers can be successfully applied for the
assessment of genetic diversity of this species. Therefore further research on
analysis of genetic diversity of cultivated enset population including divergent
production areas has to be done and the conservation capacity of institutes
both for in-situ and ex-situ should be strengthen.
Key Words: - Cultivated Ensete ventricosum, Essera, Ethiopia,
Genetic Diversity, ISSR, Kefficho,
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Keywords
Cultivated Ensete ventricosum, Essera, Ethiopia, Genetic Diversity, ISSR;Kefficho