In Vitro Regeneration of Plectranthus Edulis (Vatke) From Leaf Derived Callus
| dc.contributor.advisor | Feyissa Tileye | |
| dc.contributor.author | Aschale Nitsuh | |
| dc.date.accessioned | 2020-10-20T12:16:55Z | |
| dc.date.accessioned | 2023-11-18T09:50:14Z | |
| dc.date.available | 2020-10-20T12:16:55Z | |
| dc.date.available | 2023-11-18T09:50:14Z | |
| dc.date.issued | 2014-01-01 | |
| dc.description.abstract | Plectranthus edulis (Vatke) Agnew (syn. Coleus edulis Vatke), is a diploid, dicotyledonousplant, belonging to the Labiateae (Lamiaceae) family. It is a major source of food inmany parts of Ethiopia and also used as a traditional medicine to treat different diseases.This plant is vegetativelly propagated mainly by tubers. Vegetativelly propagated plantsare mostly affected by different diseases. These diseases reduce the yield and results inpoor quality crops. The establishment of in vitro plant regeneration system in P. eduils isof potential importance to quality improvement and genetic transformation. The studywas conducted to develop in vitro regeneration protocol for P. eduils. For this study, calliwere induced from leaf explants excised from three-week-old shoots grown on MSmedium containing different concentrations of NAA and BAP. The highest (100 %) callusinduction was obtained on MS medium supplemented with 1.5 mg/l NAA+1.0 mg/l BAPand 2 mg/l NAA+0.5 mg/l BAP. Among the different growth regulators combinationsused for multiplication, maximum (58.68±6.57) mean numbers of shoots were obtainedon 0.5 mg/l BAP in combination with 0.4 mg/l GA3. In vitro developed shoots were rootedbest with mean root number of 10.15±0.95 on MS medium supplemented with 2.0 mg/lIBA. All in vitro rooted and 78 % of microshoots were survived in the glasshouse. Thisprotocol can be used to improve this plant through in vitro screening and genetictransformation. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/12345678/22874 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Callus Induction Growth Regulator Organogenesis Regeneration | en_US |
| dc.title | In Vitro Regeneration of Plectranthus Edulis (Vatke) From Leaf Derived Callus | en_US |
| dc.type | Thesis | en_US |