In Vitro Regeneration of Plectranthus Edulis (Vatke) From Leaf Derived Callus
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Date
2014-01-01
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Addis Ababa University
Abstract
Plectranthus edulis (Vatke) Agnew (syn. Coleus edulis Vatke), is a diploid, dicotyledonous
plant, belonging to the Labiateae (Lamiaceae) family. It is a major source of food in
many parts of Ethiopia and also used as a traditional medicine to treat different diseases.
This plant is vegetativelly propagated mainly by tubers. Vegetativelly propagated plants
are mostly affected by different diseases. These diseases reduce the yield and results in
poor quality crops. The establishment of in vitro plant regeneration system in P. eduils is
of potential importance to quality improvement and genetic transformation. The study
was conducted to develop in vitro regeneration protocol for P. eduils. For this study, calli
were induced from leaf explants excised from three-week-old shoots grown on MS
medium containing different concentrations of NAA and BAP. The highest (100 %) callus
induction was obtained on MS medium supplemented with 1.5 mg/l NAA+1.0 mg/l BAP
and 2 mg/l NAA+0.5 mg/l BAP. Among the different growth regulators combinations
used for multiplication, maximum (58.68±6.57) mean numbers of shoots were obtained
on 0.5 mg/l BAP in combination with 0.4 mg/l GA3. In vitro developed shoots were rooted
best with mean root number of 10.15±0.95 on MS medium supplemented with 2.0 mg/l
IBA. All in vitro rooted and 78 % of microshoots were survived in the glasshouse. This
protocol can be used to improve this plant through in vitro screening and genetic
transformation.
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Keywords
Callus Induction, Growth Regulator, Organogenesis, Regeneration