Evaluation of Semen Parameters in Ethiopian Indigenous Bulls Kept At Kaliti, Artificial inSemination Center, Addis Ababa, Ethiopia
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Date
2004-06
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Addis Ababauniversity
Abstract
The semen of six indigenous breeds bulls (Barca, Boran, Horro and Sheko) which were kept
at the National Artificial Insemination Center (NAIC), Kaliti, Addis Ababa, Ethiopia were
evaluated for physico-morphological parameters (volume, color, mass and individual motility,
concentration, total count, percentage live and morphologically normal, total morphologically
normal and viable number), biochemical parameters (GOT, GPT, ACP, ALP, total protein)
and hormonal assay (testosterone). Semen was collected for a thirteen week period from six
indigenous bulls kept at the AI center. The collection was done once per week using an
artificial vagina and semen samples were subjected to normal physico-morphological
characterization and seminal plasma was separated and preserved at -20 0C until analysis.
The overall mean (SE) value for the indigenous bulls in the semen characteristics studied
were volume 4.84 (0.20) ml, mass motility 3.15 (0.11), individual motility 68.72 (1.37)
percent, concentration 1.54 (0.07) billion/ml, total count 7.35 (0.47) billion/ejaculate, viable
number 5.10 (0.33) billion/ejaculate, total morphologically normal number 7.12 (0.45)
billion/ejaculate, live percentage 79.73 (0.65), percent morphologic normal 94.70 (0.38),
percent normal acrosome 96.99 (0.60), GOT 1530.91 (60.15) U/L, GPT 131.99 (9.36) U/L,
ALP 3333.98 (608.84) U/L, ACP 8003.68 (716.06) U/L, total protein 7.38 (0.26) gm/dL,
testosterone 2.84 (0.3) ng/ml, head abnormality 1.87 (0.15) %, mid-piece (body) abnormality
1.92 (0.22) %, tail abnormality 1.50 (0.18) %, total abnormality 5.29 (0.38) %, major
abnormality 2.25 (0.19) %, minor abnormality 3.04 (0.28) %, acrosome defect 3.0 (0.60) %,
narrow at base 0.37 (0.09) %, abnormal contour 0.12 (0.04) %, undeveloped form 0.74 (0.15)
%, detached abnormal 0.21 (0.09) %, pear shaped 0.05 (0.02) %, small abnormal 0.05 (0.03)
%, abaxial attachment 0.57 (0.11) %, other head abnormality 0.07 (0.03) %, spermatozoa
head length 9.18 (0.03) μm, head breadth 4.61 (0.01) μm, mid-piece length 13.61 (0.02) μm,
tail length 47.56 (0.1) μm.
The semen physico-morphologic and seminal plasma biochemical parameters observed in this
study for the Ethiopian indigenous bulls are comparable to normal fertile bulls reported
elsewhere based on the evaluation of fresh semen parameters.
Key words: Ethiopia/NAIC/Indigenous breeds/physico-morphological
parameters/biochemical parameters/semen/spermatozoa
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Keywords
Ethiopia, NAICIndigenous, breeds, hysico-morphological, parameters, biochemical, parameters, semen, spermatozoa