Comparision of Hemocue301+ With Sysmex-Kx21n and Celldyn1800 Automated Hematology Analyzers for Measurement of Hemoglobin Levels in Patients at Stt. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia, 2015
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Date
2016-10
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Addis Ababa University
Abstract
Background:Hemoglobin concentration is measured routinely using automated hematologyanalyzers. Even if these automated hematology analyzers are very accurate and reliable, they areexpensive and transport of the samples to the laboratory delays the process which may delay treatment. Point of care hemoglobin analyzer like Hemocue301+ has potential to solve suchproblems. Although method comparison experiment has to be conducted when new instrumentare installed, this practice is not common in our country.
Objective:To compare hemoglobin determined by Hemocue301+, with Sysmex-kx21N and Cell-Dyn1800 methods using patients’ blood sample at St Paul’s Specialized Hospital Millennium Medical College in Addis Ababa, Ethiopia.
Methods:A facility based cross sectional study with convenient sampling method was conducted at St. Paul’s specialized teaching hospital starting from May 15 to June 2 2015. A total of117specimens for agreement study and 3 specimens for precision study were analyzed onHemocue301+, Sysmex-Kx21N, and cell-Dyn1800 hematology analyzers to compare theirHemoglobin results. This study includes patients of all age group. One sample t-test and blandand Altman plot with limit of agreement were used to make inference. Data analyses were performed using SPSS version 20 Software.
Result:The precision experiment showed within run normal range CV% for Hemocue301+,SysmexKx21N and Cell-Dyn1800 (1.7%, 1.3%, 1.8%, respectively). The one sample t-testshowed a non-significant p-value of 0.10 for Hemocue301+ and Sysmex-Kx21N and the limit ofagreement from Bland and Altman plot for 95% was (-1.07, 1.25)g/dl with only 1.7% of thecases were out of the limit of agreement. For Hemocue301+ and Cell-Dyn1800, a non-significantp- value of 0.46 and the Bland and Altman plot limit of agreement for 95% was (-1.22, 1.31)g/dlwith only 1.7%of the case were out of the limit of agreement.Hemocue301+was agreed withSysmexKx21N and Cell-Dyn1800 within 1 g/dl which was considered as clinically important limit for Hemoglobin measurement.
Conclusion: The Hemocue301+ maybe used interchangeably with Sysmex-Kx21N and Cell-Dyn1800 automated Hematology analyzers in St Paul’s Specialized Hospital Millennium Medical College Hematology laboratory
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Hemoglobin Paul’s Hospital Millennium Medical College