Isolation and Molecular Characterization of Foot and Mouth Disease Viruses in Cattle from Outbreaks Occurred in Different Parts of Ethiopia
| dc.contributor.advisor | Hika Waktole, Dr. Fanos Tadesse | |
| dc.contributor.advisor | Dr. Esayas Gelaye | |
| dc.contributor.author | Bayush, Wondimeneh | |
| dc.date.accessioned | 2020-12-25T11:46:26Z | |
| dc.date.accessioned | 2023-11-08T11:38:14Z | |
| dc.date.available | 2020-12-25T11:46:26Z | |
| dc.date.available | 2023-11-08T11:38:14Z | |
| dc.date.issued | 2020-09 | |
| dc.description.abstract | FMD is a highly contagious list A disease affecting many host species ad posing a high economic loss in different countries of the world. A cross sectional study was conducted with the aim of isolation and molecular characterization of foot and mouth disease viruses in cattle from outbreaks occurred in different parts of Ethiopia from March to April, 2020. Outbreak samples were collected for virus isolation, serotyping and molecular characterization. A total of 23 samples were collected during the outbreak and 19 were inoculated on BHK21 cell culture. Among the 19 tested samples, five (26.3%) showed cytopathic effect (CPE) after 24-48 hr of incubation. The samples were identified by using the antigen detection sandwich ELISA, only three of the samples were serotyped as serotype O and SAT1. Up on molecular characterization by RT PCR using universal primers, only two samples were positive. The two positive samples were further identified for their serotype by RT-PCR using serotype specific primers. The result revealed one of the positive sample was identified as serotype O while the other was negative for all serotype specific primers for O, A, SAT2, and SAT1. This may happen due to the higher antigenic variability of the SAT 1 serotypes which causes the failure of the primer to attach to the nucleotide. Thus, further detailed molecular analysis and serotyping of outbreak FMD viruses using sequence data for the samples which are negative by different serotype specific primers is needed. Also the detection of SAT 1 serotype circulating in the country should be an alarm to consider this serotype as vaccinal strain. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/24353 | |
| dc.language.iso | en | en_US |
| dc.subject | Ethiopia | en_US |
| dc.subject | FMD | en_US |
| dc.title | Isolation and Molecular Characterization of Foot and Mouth Disease Viruses in Cattle from Outbreaks Occurred in Different Parts of Ethiopia | en_US |
| dc.type | Thesis | en_US |