Validation of criteria for Manual Smear Review following Automated Complete Blood Counts Using the Rules Proposed by International Consensus Group for Hematology Review in St. Paul‟s Hospital Millennium Medical College, Addis Ababa, Ethiopia
| dc.contributor.advisor | Tsegaye, Aster | |
| dc.contributor.advisor | Hagos, Afework | |
| dc.contributor.author | Tefera, Belaynesh | |
| dc.date.accessioned | 2020-01-30T12:28:03Z | |
| dc.date.accessioned | 2023-11-06T08:56:27Z | |
| dc.date.available | 2020-01-30T12:28:03Z | |
| dc.date.available | 2023-11-06T08:56:27Z | |
| dc.date.issued | 2019-12 | |
| dc.description | This is to certify that the thesis prepared by Belaynesh Tefera, entitled: Validation of criteria for Manual Smear Review following Automated Complete Blood Counts Using the Rules Proposed by International Consensus Group for Hematology Review in St. Paul’s Hospital Millennium Medical College, Addis Ababa, Ethiopia and submitted in partial fulfillment of the requirements for Master of Science degree in Clinical Laboratory Sciences (Hematology and Immunohematology) complies with the regulations of the university and meets the accepted standards with respect to originality and quality | en_US |
| dc.description.abstract | Background: Microscopic assessment of a peripheral blood smear is an essential diagnostic tool in hematology laboratory. It can complement the numerical values on the automated Full Blood Count (FBC) to provide a definite diagnosis or to guide further investigation of a patient. The results of a significant proportion of specimens are flagged by the analyzers, and require confirmation by smear review. Thus, there is a need to validate the rules for smear review proposed by International Consensus Group for Hematology Review (ICGHR). Objective: To validate criteria for manual smear review following automated complete blood counts (CBC) using the rules proposed by the International Consensus Group for Hematology Review in St.Paul‟s Hospital Millennium Medical College. Methods: A prospective cross-sectional study was conducted in St.Paul‟s Hospital Millennium Medical College from September to November 2019. A total of 500 blood samples were collected from patients who are referred to hematology laboratory for CBC evaluation and who fulfilled the eligibility criteria. CBC was performed by DxH 800 Beckman coulter and manual blood smear review was done on Wright‟s stained blood film. Data was analyzed using SPSS version 25. Crosstabulation was used to determine sensitivity, specificity, Negative and positive predictive values (NPV, PPV), efficiency and review rate. Result: Out of 500 samples, 100 samples were flagged by the instrument when applying ICGHR rules. Out of these, 87 were positive when confirmed by slide review (true Flags) but 13 were negative on the smear (false positive). Moreover, 142 samples were smear positive; 55/142 smear positives were not flagged as per the ICGHR criteria (38.7%), false negative much higher than the ICGHR acceptable limit of 5% and 345 samples were true negative (normal smear and not flagged).The result showed the ICGHR rule based flagging had sensitivity of 61.3%, specificity of 96.4%, PPV 87%, NPV 86.3%, efficiency of 86.4% and review rate 20%. Conclusion: Each laboratory wishing to employ ICGHR rules have to validate them for their specific analyzer and sample population. This will ensure an acceptable false negative rate and avoid missing of clinically significant morphological abnormalities | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/20528 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Complete Blood count, Automated hematology analyzers, DxH 800 Beckman coulter , smear review criteria, Flagging, ICGHR rules | en_US |
| dc.title | Validation of criteria for Manual Smear Review following Automated Complete Blood Counts Using the Rules Proposed by International Consensus Group for Hematology Review in St. Paul‟s Hospital Millennium Medical College, Addis Ababa, Ethiopia | en_US |
| dc.type | Thesis | en_US |