The Effect of Herbal Preparations on Staphylococcus Aureus and Streptococcus Agalactiae Isolated from Clinical Bovine Masstitis
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Date
2004-06
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Addis Ababauniversity
Abstract
A study was conducted in Debre-Zeit town to determine the incidence of bovine clinical mastitis
in a purposefully selected two dairy farms and the efficacy of conventional antimicrobial drugs
and traditional herbs, from September 2003-March 2004. The objectives of the work were to
assess the invitro effect of six herbal preparations; namely, Brucea antidysentrica, Combertum
molle, Cyphostemma adenocale Persicaria senegalensis, Plantago lanceolata and Zehneria
scabra on major isolates of clinical bovine mastitis, to compare their efficacy, with conventional
antimicrobial agents that are commonly used for the treatment of bovine mastitis, and to
investigate the effect of the herbs on the growth inhibition of resistant isolates. The herbs were
collected from their natural habitats and processed and extracted with 80% methanol. Both
absolute methanol and aqueous extracts of Combertum molle were assessed foe antimicrobial
property. Milk samples from clinical cases were collected aseptically and causal agents were
identified after the severity of the diseases in each cow was categorized into Grade I-Grade III by
following standard laboratory procedures and finally sensitivity test was conducted on
Staphylococcus aureus (n=17) and Streptococcus agalactiae (n=14), which were the predominant
isolates. The incidence rate was 12.4 new clinical mastitis cases/ 100cows-month and 6.7 new
clinical mastitis cases/100cows-month at risk in EARO and Fair-field dairy farms respectively
with an overall incidence of 9 new clinical mastitis cases/100cows-month at risk. Staphylococcus
species (42.3%) and Streptococcus species (34.5%) were the major isolates from Grade I (84%)
and Grade II (16%) clinical cases. Single infection of 46.8% and mixed 37.5% and contaminated
9.4% infections were recorded with 6.3% negative cultures. Staphylococcus aureus was resistant
to Oxytetracycline (23.5%) and Penicillin G (64.7%) and Streptococcus agalactiae was resistant
to Neomycin and Streptomycin (85.7% each) and Oxytetracycline (100%). Burcae
antidysentrica, Combertum molle, Cyphostemma adenocaule and Persicaria senigalensis were
effective against susceptible and resistant isolates and among those absolute methanol extract of
Combertum molle showed a better effect on both test organisms. Plantago lanceolata and
Zehneria scabra were not showing visible inhibitory zone against test organisms. None of the
herbal extract preparations showed visible inhibitory effect on Escherichia coli. This study
indicated that mastitis is a great problem in the two dairy farms and resistant isolates are
VIII
circulating within farms. For this herbal preparations might be considered as an alternative option
for the treatment of resistant isolates of clinical bovine mastitis for the future.
Keywords: Clinical mastitis, herbal preparations, aetiological agents, Staphylococcus aureus,
Streptococcus agalactiae, sensitivity, effect, conventional antimicrobials and resistant isolates
Description
Keywords
Clinical mastitis, herbal preparations, aetiological agents, Staphylococcus aureus, Streptococcus agalactiae, sensitivity, effect, conventional antimicrobials and resistant isolates