Studies on Development of Cleaner Beam house Process, Extraction and Characterization of Collagen from Fleshing Wastes
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Date
2018-06
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Publisher
AAU
Abstract
The study presents the development of cleaner beamhouse processes to facilitate the
extraction of collagen from fleshing waste. 0.8% offer level of alkali protease in soaking
process is observed to be optimum for opening up the fibre texture of the hide, and to provide
good yield of fleshing waste. In addition to this, complete removal of hair is still an issue of
concern for unhairing using enzymes. CLRI had developed a novel protease for unhairing and
the effectiveness of this protease for unhairing in the presence of lipase as co-enzymes was
study and presented in this thesis. Experimental results indicated that 1% lipase followed by
4% protease treatment improved the unhairing efficacy. The enzymatically dehaired hide was
further analyse by SEM, and the complete removal of hair in the experimental process (1%
lipase followed by 4% protease) is further established. The reduction in pollution load
parameters viz., BOD, COD, TOC, TDS and TSS is 89.375%, 88.57%, 85.79%, 47.37% and
48.57%, respectively with reference to conventional unhairing process. The leathers
processed from optimized soaking and unhairing system showed comparable strength
characteristics with reference to leather processed with chemical unhairing system. Hence a
total bio-based beamhouse processing in leather manufacture was feasible. Fleshing from
conventional lime and green fleshing wastes were used for the extraction of collagen, which
is high value protein that can be used for a wide range of applications viz., tissue engineering,
cosmetic, food, and wound healing and pharmacy application. Extraction of collagen had
been carried out using acetic acid solubilisation and it is observed that collagen yield obtained
from green fleshing (96.53%) is higher than lime fleshing (84.87%). FTIR, SDS-PAGE, CD
spectra and thermal melting experiments are used for the characterization of collagen. It was
confirmed that the extracted collagen from lime/green fleshing wastes were type I and also
found to have similar properties. This study clearly establishes the development of cleaner
beamhouse process and effective recovery of collagen from fleshing waste.
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Keywords
Beam house Process, Cleaner, Collagen, Fleshing Wastes