ESCHERICHIA COLI O157:H7: PREVALENCE AND SOURCES OF CONTAMINATION OF CATTLE MEAT AT MUNICIPAL ABATTOIR AND BUTCHERIES AS WELL AS ITS PUBLIC HEALTH IMPORTANCE IN ADDIS ABABA, ETHIOPIA
| dc.contributor.advisor | Dr. Ashenafi Feyissa, Dr. AkafeteTeklu | |
| dc.contributor.author | WOYNSHET, HAILE | |
| dc.date.accessioned | 2020-06-18T11:13:43Z | |
| dc.date.accessioned | 2023-11-08T11:38:12Z | |
| dc.date.available | 2020-06-18T11:13:43Z | |
| dc.date.available | 2023-11-08T11:38:12Z | |
| dc.date.issued | 2014-06 | |
| dc.description.abstract | Escherichia coli O157: H7 is recognized as an important cause of diarrhea, hemorrhagic colitis and hemolytic-uremic syndrome worldwide. The present study was conducted from November 2013 to April 2014 on 150 apparently healthy slaughtered cattle at municipal abattoir, 115 different butcher houses and 50 stool samples from two health centers in Addis Ababa, Ethiopia with the objectives of establishing prevalence of E. coli O157: H7 on the carcasses at the municipal abattoir and meat presented for sale in butcher shops, providing information as to the major sources of carcass contamination in abattoir and butcher shops so that to forward strategies in minimizing the contamination and to study the public health importance of the organism in the study area. Different samples was taken for this study; skin swab, fecal samples, intestinal mucosal swabs and the inside and outside part of carcasses as well as carcass in contacts such as workers hands, knife, transporter clothes and vehicles from the abattoir and carcass swab, cutboard, butcher men hand and knife from butcher houses. In addition, stool samples from suspect individuals visiting in both Kazanchis and Kaliti health centers were collected. All the samples were then transported with buffered peptone water to the Microbiology Laboratory of College of Veterinary Medicine, Addis Ababa University for isolation and identification of E. coli O157:H7. After enrichment of samples with Triptone soya Broth supplemented with novobiocin and concentration of E. coli O157:H7 using Immuno Magnetic Separation it was inoculated on Sorbitol MacConckey agar supplemented with antibiotics, Cefixime and Potassium Telurite. Non-Sorbitol fermenting colonies were tested on latex agglutination test.From all 1380 samples tested only 10(0.72%) were found to be positive of E. coli O157:H7.From these 10(0.72%) positive samples 8(1.03) were from abattoir and 2(0.43%) were from butcher houses. No positive isolate were found from both health centers. All E. coli O157:H7 isolates were then checked for their susceptibility pattern by 10 selected antibiotics. The isolated strains were found to be susceptible (100%) to Kanamycine, Chloramphenicol, Ciprofloxacin, Sulfamethoxazole-trimithoprim and Nalidixicacid and also a Multidrug resistance to all 10 drugs was detected for all strain. To prevent contamination Hazard Analysis of Critical Control Points (HACCP) should be applied from farm to fork. | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/21782 | |
| dc.language.iso | en | en_US |
| dc.subject | Addis Ababa | en_US |
| dc.subject | CT-SMAC | en_US |
| dc.subject | E.coli O157:H7 | en_US |
| dc.title | ESCHERICHIA COLI O157:H7: PREVALENCE AND SOURCES OF CONTAMINATION OF CATTLE MEAT AT MUNICIPAL ABATTOIR AND BUTCHERIES AS WELL AS ITS PUBLIC HEALTH IMPORTANCE IN ADDIS ABABA, ETHIOPIA | en_US |
| dc.type | Thesis | en_US |