STUDIES ON THE PEREVALENCE, ANTIBIOGRAM, ASSESSMENT OF RISK FACTORS AND PUBLIC HEALTH SIGNIFICANCE OF STAPHYLOCOCCUS AUREUS IN BEEF AND ENVIRONMENT AT ADDIS ABABA
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Date
2014-06
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Abstract
A cross-sectional study was conducted from October 2013-April 2014 to determine the prevalence of Staphylococcus aureus from Addis Ababa abattoir and retail shops from beef Carcasses and equipment to determine antibiotic resistance profiles of the isolates found in and around Addis Ababa. A total of 888 samples comprising of 384 abattoir and 384 retail shops. carcasses swabs were in the transport medium using systematic random sampling. The swab samples were also obtained from abattoir equipment (n=60) and retail shop equipment n=(60). In addition, questionnaire survey was conducted to assess the hygienic practice during working time and possible risk factors regarding the contamination of meat. Staphylococcus aureus was isolated and identified using standard bacteriological techniques. The prevalence of Staphylococcus aureus in abattoir, retail shops and equipments was 9.3%, 19.7%, 17.5% respectively. Consequently, statistically significant association (p= 0.00) was observed in the prevalence of S. aureus between abattoir and retail shop Antimicrobial susceptibility test was also conducted on 133 isolates of S.aureus, using the disc diffusion susceptibility method. In this study, varying level of resistance of S. aureus was against bacitracin, methicillin, neomycin, and teteracyclin 95.1% was noticed. The susceptibility pattern indicated that S. aureus isolates exhibited varying level of resistance to two or more antimicrobial agents. Preventive measures to avoid the presence of pathogenic Staphylococcus isolates by adopting the good hygienic practices during slaughtering and handling of beef carcasses are emphasized.
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Abattoir, Antimicrobia, susceptibility, Beef, Carcass, Isolation, prevalence