Establishment of community based hematological reference intervals among apparently healthy adults (18-60 years) in Mekelle, Tigrai, Northern Ethiopia from December 2018-May 2019: a cross sectional study
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Date
2019-06
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Addis Abeba University
Abstract
Background: the medical laboratory service is a key component of the overall health system.
The service provides useful laboratory data to prevent, control, treat and follow up for patients.
Locally derived reference interval (RI) is recommended to interpret laboratory data because there
are various physiological and environmental factors which affect it. There was no reference
interval established for hematological parameters in Mekelle, Tigrai, Northern Ethiopia.
Objective: Establishment of community based hematological reference intervals among
apparently healthy adults (18-60 years) in Mekelle, Tigrai, Northern Ethiopia from December
2018-May 2019: a cross sectional study.
Method: a cross-sectional study was employed in 344 apparently healthy adult (18-60)
individuals who live at least 5 years in Mekelle city, Tigrai, northern Ethiopia from December
2018-2019. About 4ml whole blood, stool and urine samples were collected. Hematological
parameters were analyzed by Sysmex Kx-21 at Wukro hospital. Peripheral blood morphology
and malarial also were screened. The urine analysis, stool wet mount and Kato Katz were
examined at collection sites and the formol-ether concentration was examined at the Tigrai
health research institute. Data were entered and analyzed using SPSS version 20. The 2.5
and
97.5
th
percentiles were calculated using non-parametric methods to determine 95% RI.
Result: The established reference interval for hematological parameters were White blood cell:
3-10x10
9
/L; lymphocyte percentage :19.6-57.6%; neutrophil percentage :24.91-70.3% for both
sexes (p>0.005); Red blood cell parameters were (p<0.001): 4.25-5.46 versus 4.7 -6.09 x 10
/L,
hemoglobin :12.4-15.5 versus 13.9-17.85 g/dl; hematocrit: 38-48 versus 41.6-53.2 % for males
and females respectively, but platelet was higher in females than males 158.3 -399.5 versus 142345.1
x
10
9
/L.
Conclusion: The established hematological reference interval for Mekelle city was different
from other reported studies. So, this finding is important to diagnosis, treatment and follows up
of the clients of the Mekelle, Tigrai, Northern Ethiopia. There was statistically significant
difference based on gender difference on red blood cell, hemoglobin, hematocrit, platelet and
mixed percentage.
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12
Description
Keywords
Apparently healthy adults, CBC, Hematology, Reference interval, Ethiopia