The Effects of Culture Conditions on in Vitro Propagation of Korarima (Aframomum Corrorima (Braun) P.C.M. Jansen)
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Date
2020-06-22
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Addis Ababa University
Abstract
Aframomum corrorima is one of the important spice and medicinal plants of the family Zingiberaceae which can be propagated from its seed and rhizome. Conventionally, propagation of A.corrorima by rhizome causes for genetic erosion and it is a time consuming process. Hence, plant tissue culture has played a very important role to clone the endangered plant species with desirable traits urgently. The present study was conducted to investigate the effects of culture conditions on in vitro propagation of A.corrorima using MS liquid media. Surface sterilization of seeds in 70% ethanol for 3 minutes then followed by dipping in 25% sodium hypochlorite for 25 minutes gave the best disinfected seeds and the percentages of seed germination and shoot initiation obtained on MS media were 93.5% and 96.25% ,respectively. In the sucrose containing MS media, 3% gave the highest number of shoots (3.10±0.15), leaves (8.77±0.13), roots (9.03±0.72) and shoot length (3.39±0.63 cm) per explant/shoot. Out of different pH levels, pH 5.8 gave the best result regarding the number of shoots (3.20±0.13), leaves (11.40±0.27) and length of shoots (4.08±0.13cm) and root number (9.03±0.72) of korarima. In another MS liquid culture media experiment, maximum shoot number (3.03 ±0.21) and shoot length (3.17 ± 0.12 cm) were recorded in full MS strength medium. The best root number (14.47± 0.96) and root length (6.30 ±0.11cm) were obtained on half MS strength medium at pH 5.8, with sucrose supplied at a concentration of 3% and 0.5 mg/l NAA (-naphthaleneacetic acid) when shoots were rooted. Acclimatization in greenhouse resulted in 94. 3 % with a mixture of forest soil, sand and farmyard in the ratio of 2:1:1. The result of this study can be used in the future protocol optimization and mass propagation of A. corrorima.
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Keywords
Acclimatization, Aframomum Corrorima, In Vitro Propagation, Ms Salt Strength, Sucrose Concentration