An Experimental Study on the Effect of Crude Extracts of Moringa Stenopetala and Artemisia Absinthium in Trypanosoma Congolense Infected Mice
| dc.contributor.advisor | Getachew Terefe | |
| dc.contributor.author | Tsegabirhan K.yohannes | |
| dc.date.accessioned | 2026-08-12T09:24:26Z | |
| dc.date.available | 2026-08-12T09:24:26Z | |
| dc.date.issued | 2011-06-01 | |
| dc.description.abstract | An experimental study was conducted to determine the in vivo anti-trypanosomal effect of water and methanol extracts of the leaves of Moringa stenopetala and aerial parts of Artemisia absinthium against Trypanosoma congolense isolated from Ghibe valley. Thirty-six Swiss white male mice aged between 8 - 12 weeks were divided into six experimental groups of six animals. Water and methanol extracts of the two plants were prepared. T. congolense was isolated from cattle at Ghibe valley (western central Ethiopia) and propagated on mice. All mice groups received I x 105 trypanosomes in 0.2 ml of blood. The plant extracts were given orally to four groups (A. absinthium with water extract, and with methanol extract, M stenopetala with water extract, and with methanol extract) at 400mg/kg body weight for 7 consecutive days. One group remained as distilled water treated control and the other as diminzene aceturate treated control. The effect of the extracts on levels of parasitaemia, body weight, pcv and mice survival was monitored for 25 days. All treatments have significantly reduced the parasitaemia of mice compared to the water treated control (P<O.O I). The effect of all plant extracts in the reduction of parsitaemia in infected mice was comparable to that of diminazene aceturate (P>O.05). There was no significant difference in the reduction of parasitaemia between groups treated with: water extracts of both test plants, methanol extracts of both test plants, water and methanol extracts of A. absinthium, and water and methanol extracts of M stenopetala. The results clearly indicate that groups tTeated with the plant extracts and diminazene aceturate showed significantly lower parasitaemia, higher body weights, pcv and survival rates than the water treated control group (P<O.05 in all cases). The effect of all plant extracts on the reduction of parasitaemia and improvement of body weights, pcv and survival of infected mice was comparable to that of diminazene aceturate. However, group receiving water extract of A. absinthium had significantly higher body weight (mean: 35.9±0.3g, cI: 35.1-36.7g) than water extract of M stenopetala (mean: 34.7±0.4g, cI: 33.7-35.7g) (P<O.05). Similarly, methanol extract of A. absinthium resulted in significantly higher body weight (mean: 36.5±0.3g, cI: 35.8-37.lg) than methanol extract of M stenopetala (mean: 34.8±0.3g, el: 34.1-35.4g) (P<O.OI). There was no significant difference in the levels of parasitaemia and pcv between groups treated with: water extracts of both test plants, methanol extracts of both test plants, water and methanol extracts of A. absinthium, and water and methanol extracts of M stenopetala. It can be concluded that the two plants have excellent potential against pathogenic trypanosomes (T. congolense) of livestock importance by reducing the levels of parasitaemia, maintaining good pcv and body weight, and prolonging the lives of intected animals. Overall, methanol extracts and A. absinthium showed better performance compared to water extracts and M stenopetala. Additional study is recommended using various doses and extraction methods before any further application. | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/8849 | |
| dc.language.iso | en | |
| dc.publisher | Addis Ababa University | |
| dc.subject | antitrypanosomal activity | |
| dc.subject | Artemisia absinthium | |
| dc.subject | Moringa stenopetafa | |
| dc.subject | mice | |
| dc.subject | parasitaemia | |
| dc.subject | PCv | |
| dc.subject | survival time | |
| dc.subject | Trypanosoma congolense | |
| dc.subject | weight | |
| dc.title | An Experimental Study on the Effect of Crude Extracts of Moringa Stenopetala and Artemisia Absinthium in Trypanosoma Congolense Infected Mice | |
| dc.type | Thesis |