Serum Total and Placental Alkaline Phosphatas at Second and Third Trimesters of Normal Pregnant Mothers from July to Nov 2015 at Zewditu Memorial Hospital, Addis Ababa, Ethiopia
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Date
2016-07
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Addis Ababa University
Abstract
Background: Alkaline phosphatase (ALP) (EC 3.1.3.1.) is an enzyme which catalyzes the
hydrolysis of phosphate esters at an alkaline PH. Placental alkaline phosphatase isoenzyme
(PLAP) has several vital roles during pregnancy for supporting fatal growth and development.
The change of PLAP may be associated with high blood pressure, preterm delivery and other
complication. It could be an indicator of overall placental performance and pregnancy outcomes.
Therefore, there is the need to determine and proper interpretation in our local setup.
Objectives: To evaluate serum total and placental alkaline phosphatase at second and third
trimesters in normal pregnant mothers in our local setup.
Method: Across-sectional study was done on randomly selected 333 consenting apparently
healthy pregnant mothers at Zewditu Memorial Hospital in Addis Ababa, Ethiopia. Maternal
demographic characteristic was obtained through questionnaires and medical chart review. The
TALP and PLAP were analyzed by Human 4-Nitrophenylphosphatas (4-NPP) substrate. The
data was analyzed using the SPSS software, version 21. The data was assessed using non-
parametric analysis; hence the data was found to be skewed. P< 0.05 was considered statistically
significant.
Result: The median of TALP and PLAP in second trimester were ( 163 IU/L) and (25 IU/L)
respectively while in third trimester were (334 IU/L) and (141IU/L) respectively and at term
were (449 IU/L) and ( 230 IU/L) respectively (P< 0001). Strong positive correlation observed
between TALP (r2 = 0.805, p<0.001) and PALP with gestational age (r2 = 0.805, P< 0.001). Chi-
square showed positive association with blood group and blood pressure (P<0.05).
Conclusion and Recommendation
There was significant increasing serum TALP and PLAP with advancing gestational age. There
was a peak levels at term. Strong positive correlation observed between TALP and PALP with
gestational age. Also, blood group and blood pressure were significantly associated. Thus, it
could be used as an easy accessible and affordable biomarker for monitoring the status of
pregnancy.
Keywords:
Total
and
Placental
alkaline
phospatase,
pregnant
mothers
Trimester
Description
Keywords
Total and Placental alkaline phospatase, pregnant mothers Trimester