Prevalence of Bacterial Contamination and Antimicrobial Susceptibility Pattern among Blood and Blood Components Collected with and without Diverging Method at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia
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Date
2018-11
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Addis Ababa University
Abstract
Background: Transfusion of bacterial contaminated blood and blood components couldbe a cause of morbidity and mortality. Understanding the mechanism of blood contamination is important in developing infection control strategy.
Objectives: To determine prevalence of bacterial contamination and antimicrobial susceptibility pattern among blood and blood components collected with and withoutdiverging method.
Methods: A comparative cross-sectional study was done from January to April 2018 on blood and blood components collected with and without diverging method at the studyarea. A total of 376 blood sample of blood and blood components were taken. 3 ml ofsamples from each was inoculated on 15 ml Brain Heart Infusion followed by incubationfor seven days at 37oC then after subculture on Sheep Blood Agar, MacConkey Agar andChocolate Agar. Finally, the antimicrobial susceptibility test was done for each isolate.Data were entered and analyzed using SPSS version 20 software. P ≤ 0.05 wasstatistically significant.
Results: The overall prevalence of bacterial contamination among blood and blood components were 4.5% (N= 17/376). The prevalence of bacterial contamination of blood and blood components collected with the non-diverging and diverging method was 7.4%(n= 14/188) and 1.6 %( n= 3/188) respectively with P value of 0.05. Staphylococcusepidermidis were the most dominate isolates. Gram positive isolates showed more than74% sensitive for antibiotics and also became more than 9% resistant. Most gramnegative isolates became sensitive but Pseudomonas aeruginosa showed resistant for Gentamicin.29.4% (n= 5/17) isolated bacteria were multidrug resistant.
Conclusion: There was a difference between bacterial contamination in blood and blood components collected between diverging and non-diverging blood collection methods.
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Bacteria contamination, Diverging and non-diverging method, Blood culture