Prevalence of Bacterial Contamination and Antimicrobial Susceptibility Pattern among Blood and Blood Components Collected with and without diverging method at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia

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Addis Ababa University


Background: Transfusion of bacterial contaminated blood and blood components could be a cause of morbidity and mortality. Understanding the mechanism of blood contamination is important in developing infection control strategy. Objectives: To determine prevalence of bacterial contamination and antimicrobial susceptibility pattern among blood and blood components collected with and without diverging method. Methods: A comparative cross-sectional study was done from January to April 2018 on blood and blood components collected with and without diverging method at the study area. A total of 376 blood sample of blood and blood components were taken. 3 ml of samples from each was inoculated on 15 ml Brain Heart Infusion followed by incubation for seven days at 37oC then after subculture on Sheep Blood Agar, MacConkey Agar and Chocolate Agar. Finally, the antimicrobial susceptibility test was done for each isolate. Data were entered and analyzed using SPSS version 20 software. P ≤ 0.05 was statistically significant. Results: The overall prevalence of bacterial contamination among blood and blood components were 4.5% (N= 17/376). The prevalence of bacterial contamination of blood and blood components collected with the non-diverging and diverging method was 7.4% (n= 14/188) and 1.6 %( n= 3/188) respectively with P value of 0.05. Staphylococcus epidermidis were the most dominate isolates. Gram positive isolates showed more than 74% sensitive for antibiotics and also became more than 9% resistant. Most gram negative isolates became sensitive but Pseudomonas aeruginosa showed resistant for Gentamicin.29.4% (n= 5/17) isolated bacteria were multidrug resistant. Conclusion: There was a difference between bacterial contamination in blood and blood components collected between diverging and non-diverging blood collection methods.



Bacteria contamination, Diverging and non-diverging method, Blood culture