Prevalence of Bacterial Contamination and Antimicrobial Susceptibility Pattern among Blood and Blood Components Collected with and without diverging method at Armed Forces Comprehensive Specialized Hospital, Addis Ababa, Ethiopia
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Date
2018-11
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Addis Ababa University
Abstract
Background: Transfusion of bacterial contaminated blood and blood components could
be a cause of morbidity and mortality. Understanding the mechanism of blood
contamination is important in developing infection control strategy.
Objectives: To determine prevalence of bacterial contamination and antimicrobial
susceptibility pattern among blood and blood components collected with and without
diverging method.
Methods: A comparative cross-sectional study was done from January to April 2018 on
blood and blood components collected with and without diverging method at the study
area. A total of 376 blood sample of blood and blood components were taken. 3 ml of
samples from each was inoculated on 15 ml Brain Heart Infusion followed by incubation
for seven days at 37oC then after subculture on Sheep Blood Agar, MacConkey Agar and
Chocolate Agar. Finally, the antimicrobial susceptibility test was done for each isolate.
Data were entered and analyzed using SPSS version 20 software. P ≤ 0.05 was
statistically significant.
Results: The overall prevalence of bacterial contamination among blood and blood
components were 4.5% (N= 17/376). The prevalence of bacterial contamination of blood
and blood components collected with the non-diverging and diverging method was 7.4%
(n= 14/188) and 1.6 %( n= 3/188) respectively with P value of 0.05. Staphylococcus
epidermidis were the most dominate isolates. Gram positive isolates showed more than
74% sensitive for antibiotics and also became more than 9% resistant. Most gram
negative isolates became sensitive but Pseudomonas aeruginosa showed resistant for
Gentamicin.29.4% (n= 5/17) isolated bacteria were multidrug resistant.
Conclusion: There was a difference between bacterial contamination in blood and blood
components collected between diverging and non-diverging blood collection methods.
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Keywords
Bacteria contamination, Diverging and non-diverging method, Blood culture