Molecular Characterization and Drug Resistance Pattern of Mycobacterium Tuberculosis Complex among Confirmed Extrapulmonary Tuberculosis Cases in Addis Ababa, Ethiopia

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dc.contributor.advisorDesta Kassu
dc.contributor.advisorAbera Dessie
dc.contributor.advisorKebede Abebaw
dc.contributor.advisorHailu Habteyes
dc.contributor.authorDiriba Getu
dc.date.accessioned2020-11-29T07:11:16Z
dc.date.accessioned2023-11-06T08:56:40Z
dc.date.available2020-11-29T07:11:16Z
dc.date.available2023-11-06T08:56:40Z
dc.date.issued2020-06
dc.description.abstractBackground: Molecular characterization and drug-resistant of Mycobacterium tuberculosis is avaluable tool in understanding the pathogenesis, diagnosis treatment, and prevention oftuberculosis. However, there is limited information on molecular characterization, and drug resistantpatterns of Mycobacterium tuberculosis in patients with extrapulmonary tuberculosis inEthiopia. Objective: This study aimed to determine the molecular characteristic and drug resistancepatterns of Mycobacterium tuberculosis complex among confirmed extrapulmonary tuberculosiscases in Addis Ababa, Ethiopia Methods: A laboratory-based cross-sectional study was conducted among confirmedextrapulmonary tuberculosis cases in Addis Ababa, Ethiopia in a period between November2019 and April 2020. Drug susceptibility test was performed using BACTEC-MGIT 960 and Line probe assay. Strain identification was performed using the Geno-Type MTBC andspoligotyping technique. Data were entered into the international spoligotyping database toassess the spoligotype patterns of Mycobacterium tuberculosis. Data was analyzed by SPSSversion 23 and participants’ characteristics were presented by number and proportion. Results: Of 151 Mycobacterium tuberculosis complex isolates, 29 (19.2%) were resistant to atleast one drug, and 14 (9.3%) were identified as multidrug-resistant tuberculosis. Besides,21.4% (3/14) of isolates had pre-extensively drug-resistant tuberculosis. S315Tl (11.9% (18/151)was the most common isoniazid mutation observed in the katG gene. The spoligotyping of the151 isolates resulted in 41 different spoligotype patterns. The most abundantly represented SITswere: SIT149 21.2% (33/151), SIT53 14.6% (22/151) and SIT26 9.6% (14/151). The mostpredominant lineage was T family 55.0% (83/151), Central Asia Strain 19.2% (29/151) andHaarlem consisted of 8.0% (12/151). Also, 5 (3.3%) isolates had no matching with the database. Conclusion: The present study showed a high proportion of multidrug-resistant tuberculosis andpre-extensively drug-resistant tuberculosis among EPTB patients. The strain was mostly groupedinto SIT149, SIT53, and SIT26. A large scale is required to define the molecular characteristicsand drug resistance of Mycobacterium tuberculosis in extrapulmonary tuberculosis patients. en_US
dc.identifier.urihttp://etd.aau.edu.et/handle/123456789/23693
dc.language.isoen_USen_US
dc.publisherAddis Abeba Universityen_US
dc.subjectExtrapulmonary Tuberculosis, Molecular typing, Mutations, multidrug-resistant tuberculosis and pre-extensively drug-resistant tuberculosisen_US
dc.titleMolecular Characterization and Drug Resistance Pattern of Mycobacterium Tuberculosis Complex among Confirmed Extrapulmonary Tuberculosis Cases in Addis Ababa, Ethiopiaen_US
dc.typeThesisen_US

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