Rhizobial Status and Strain Identification of Some Pulse Fields at Ankober, Keyt, Mehal-Meda and Molale (Northern Shewa)

dc.contributor.advisorBekele Endashaw (Professor)
dc.contributor.advisorAssefa Fassil (PhD)
dc.contributor.authorAdamu, Aynabeba
dc.date.accessioned2018-06-21T14:01:19Z
dc.date.accessioned2023-11-08T16:32:38Z
dc.date.available2018-06-21T14:01:19Z
dc.date.available2023-11-08T16:32:38Z
dc.date.issued1999-05
dc.description.abstractThis study oonsiders the rhizobial status of some localities in the Northern Shewa Zone, where faba bean yield has deoreased. Composite soil samples were taken from representative areas of two yield depleted areas, Molale and Mehal-Meda and two yield sustained areas, Keyt and Ankober.Soil analysis was done on the four soil samples to know their nutrient content and status of rhizobial population. It was found that the m.imbe~ of rhizobia per gram of yield depleted area soils was very low where as it was very high in the yield sustained area soils. The phosphorus and potassium cOhtentof Molale and Mehal-Meda soils was also lower than that of Ankober and Key! soils. However, the nitrogen content of all the four soil samples was high. Also four Rhizobium leguminosarum strains were isolated from these soil samples by planting the host plant faba bean. The isolates were identified as different strains by using PCR-based amplification of specific DNA fragments of nitrogen fixing promoter region of the Rhizobium genome. The relative symbiotic effectiveness of the four isolates and one exotic strain Tal 1397N was determined in sand culture. The results showed that all the four local isolates and the exotic strain haven't caused any significant improvement from the control in any of the growth parameters except nodule fresh weight. Pot experiment was conducted to know the effect of inOCUlation by the local isolates and the exotic strain (Tal 1397N) and nitrogen and phosphorus fertilizers (urea and DAP) on the growth of faba bean.en_US
dc.identifier.urihttp://etd.aau.edu.et/handle/123456789/2795
dc.language.isoenen_US
dc.publisherAddis Ababa Universityen_US
dc.subjectBiologyen_US
dc.titleRhizobial Status and Strain Identification of Some Pulse Fields at Ankober, Keyt, Mehal-Meda and Molale (Northern Shewa)en_US
dc.typeThesisen_US

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