Rhizobial Status and Strain Identification of Some Pulse Fields at Ankober, Keyt, Mehal-Meda and Molale (Northern Shewa)

No Thumbnail Available

Date

1999-05

Journal Title

Journal ISSN

Volume Title

Publisher

Addis Ababa University

Abstract

This study oonsiders the rhizobial status of some localities in the Northern Shewa Zone, where faba bean yield has deoreased. Composite soil samples were taken from representative areas of two yield depleted areas, Molale and Mehal-Meda and two yield sustained areas, Keyt and Ankober.Soil analysis was done on the four soil samples to know their nutrient content and status of rhizobial population. It was found that the m.imbe~ of rhizobia per gram of yield depleted area soils was very low where as it was very high in the yield sustained area soils. The phosphorus and potassium cOhtentof Molale and Mehal-Meda soils was also lower than that of Ankober and Key! soils. However, the nitrogen content of all the four soil samples was high. Also four Rhizobium leguminosarum strains were isolated from these soil samples by planting the host plant faba bean. The isolates were identified as different strains by using PCR-based amplification of specific DNA fragments of nitrogen fixing promoter region of the Rhizobium genome. The relative symbiotic effectiveness of the four isolates and one exotic strain Tal 1397N was determined in sand culture. The results showed that all the four local isolates and the exotic strain haven't caused any significant improvement from the control in any of the growth parameters except nodule fresh weight. Pot experiment was conducted to know the effect of inOCUlation by the local isolates and the exotic strain (Tal 1397N) and nitrogen and phosphorus fertilizers (urea and DAP) on the growth of faba bean.

Description

Keywords

Biology

Citation

Collections