Antidiarrheal and Antispasmodic Activities of Essential Oil of Myrtus Communis L

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Addis Ababa University


The essential oil of Myl'/us communis (EOMC) was evaluated for its antid iarrheal and antispasmodic potential against isolated guinea ileum (OPI), ex-vivo antispasmodic model; normal and castor oil-induced intestinal transit in mice; castor oi l-induced diarrhea in mice and prostaglandin induce enteropooling in rats. Atropine was used in OPI and normal intestinal transit test as a positive control, whereas loperamide was used in the castor oil-induced intestinal transit and castor oil-induced antidiarrheal test. EOMC inhibited normal intestinal transit siginificantly (p<0.05) and the effect was comparable with that of atropine. All doses (100, 200 and 400 mg/kg) of the oil employed showed significant antidiarrheal and antienteropooling activities which was comparable with that of the positive control loperamide. Different concentrations of the essential oil were used in the presence of agonist (Acetylcholine) in OP! as contraction stimulator in ex-vivo. The oil exhibited significant reductions in Acetylcholine-induced contractions of OP!. The agonist-induced contractions of OP] were greatly reduced by both doses of 50 fig/ml (p<0.01) and 100 fig/ml (p<0.01), suggesting a powerful spasmolytic property of the oil. The effect produced showed that the oi l is much more efficacious than atropine (6.66 xl 0.9 M) in ex-vivo model. The oil appears to be more efficacious in ex-vivo than in vivo which may be due to the difference in physiological conditions that exists between the two systems. This study suggested that the essential oil of M. communis possesses spasmolytic and antidiarrheal properties which are likely to be due to the a-pinene and linalool present in the oil. The spasmolytic and antidiarrheal mechanisms might be in part mediated via Ca + -channel blockage. The results obtained in this study also SUpp0l1 the traditional use of the plant for stomach pains, and diarrhea. However, fUl1her study should be conducted in order to determine the exact mechanism (s) of action of the oil and also to characterize the constituents responsible for the activity observed.Key words: M. communis, essential oil, antidiarrheal, antispasmodic, antienteropooling



communis, essential oil, antidiarrheal, antispasmodic, antienteropooling IX