Molecular Characterization of Glucose-6-Phosphate Dehydrogenase Deficiency Specific Variants among Selected Populations in Malaria Endemic areas of Ethiopia
| dc.contributor.advisor | Assefa Assefu | |
| dc.contributor.author | Getachew Abebe | |
| dc.date.accessioned | 2018-06-29T06:46:15Z | |
| dc.date.accessioned | 2023-11-06T08:57:34Z | |
| dc.date.available | 2018-06-29T06:46:15Z | |
| dc.date.available | 2023-11-06T08:57:34Z | |
| dc.date.issued | 2015-11 | |
| dc.description.abstract | Background: Glucose 6-phosphate dehydrogenase deficiency (G6PDd) is an X-linked hereditarygenetic defect, affects an estimated 400 million people worldwide. Severe clinical manifestationassociated with G6PDd (e.g., chronic hemolytic anemia) depends on the type of G6PD molecularvariants and exposure to hemolytic triggers (e.g., antimalarial like Primaquine). However, scarcestudies on G6PDd renders the use of Primaquine for effective therapeutic treatment of malaria. Objective: To determine the availability and characterize selected molecular variants of G6PDdspecific genes among selected populations in malaria endemic area of Ethiopia. Method: A cross sectional study was conducted among selected populations in malaria endemicareas of Ethiopia from July 30, 2014 to January 30, 2015. A total of 523 dried blood spot samples were randomly selected from stored samples of national malaria indicator survey of 2011.Polymerase chain reaction and restricted fragment length polymorphism technique was applied tocharacterize G6PDd variants as G6PD*A, G6PD*A- and/or G6PD*Mediterranean. Binary logisticregression was applied to see association (P<0.05 is significant) among different parameters. Result: Of 523 studied dried blood spot samples, 514 (98.28%) had G6PD genotype available,among which G6PDd were detected on 46 (9.0%) samples. G6PD*A (100%) was the onlygenotype characterized, while neither G6PD*A- nor G6PD*Mediterranean genotypes weredetected. Of all 46 (9.0%) G6PD*A mutation, 25 (4.9%) were male hemizygous, 4 (0.8%) werehomozygous females and 17 (3.3%) were heterozygous females. The result also showed G6PDdprevalence variation among regions with 12.06% in Southern Nations Nationalities Peoples,10.62% in Tigray, 8.51% in Somali, 6.41% in Amhara and 5.26% in Afar. However; there was nostatistical significant difference between G6PDd and regions (P>0.05). Conclusion: G6PD*A variant was the only G6PDd genotype detected in this study. G6PD*Avariant has almost (90%) the same enzymatic activities with the wild type. Therefore; this resultsupports the safe use of primaquine, especially the single low dose for transmission interruptionof Plasmodium falciparum gametocyte and radical cure of Plasmodium vivax, as a part of malaria elimination toolkit, among selected populations in malaria endemic areas of Ethiopia. Key terms: Glucose 6-phosphate Dehydrogenase deficiency, Polymerase Chain Reaction,Restriction Fragment Length Polymorphism, Malaria, Primaquine, Ethiopia | en_US |
| dc.identifier.uri | http://etd.aau.edu.et/handle/123456789/4843 | |
| dc.language.iso | en | en_US |
| dc.publisher | Addis Ababa University | en_US |
| dc.subject | Glucose 6-phosphate Dehydrogenase deficiency Polymerase Chain Reaction Restriction Fragment Length PolymorphismMalari Primaquine | en_US |
| dc.title | Molecular Characterization of Glucose-6-Phosphate Dehydrogenase Deficiency Specific Variants among Selected Populations in Malaria Endemic areas of Ethiopia | en_US |
| dc.type | Thesis | en_US |