Morphometrical, Pathological and Bacteriological Study of Mammary Gland of Cows With Subclinical and Clinical Mastitis in Selected Farms and Abattoirs in Central Ethiopia

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Date

2016-06

Authors

Meaza, Mandefrot

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Publisher

Addis Ababauniversity

Abstract

The cross sectional study was conducted from November 2015 to May 2016 in Bishoftu, Addis Ababa and Adama areas to evaluate cow’s udder morphometrical changes in relation to mastitis, mammary gland lesion characterization and isolation and characterization of aerobic bacteria from lesions. A total of 252 dairy cow from selected dairy farms in Bishofu and 72 udders from Addis Ababa and Adama municipality abattoirs were included in the study. The study revealed that the overall prevalence of mastitis was found to be 44.44% with clinical and sub-clinical mastitis accounting 16.11% and 36.67%, respectively. Morphometrically, cows with longer and thicker teats, greater distance between hind teats, Longer glandular longitudinal length and shorter teat end to floor distance had higher frequency of subclinical mastitis and the difference was statistically significant (P<0.05). However, udder and teat end shape had no significant association (P>0.05) with subclinical mastitis. Distance between hind teats and front udder height had direct statistically (p<0.05) association with somatic cell count. Out of 217 quarter milk samples cultured, 147 (67.74%) were positive for single colony, 46 (21.2%) for mixed bacterial growth and 24 (11.06%) were negative for bacterial growth. Predominant aerobic bacteria isolated were Coagulase Negative Staphylococci 66 (34.2%) followed by S. aureus 63 (32.8%) and E.coli 40 (20.7%). On the other hand, from culture result of 53 mammary gland tissue samples collected from abattoirs, the major isolated bacteria were E. Coli (35cases), streptococci (32 cases), S. aureus (23 cases), Coagulase Negative Staphylococci (14 cases),, and S. hyicus (11 cases). Tissue sections from abattoir showed glandular epithelial degeneration and necrosis, atrophy of alveoli and the glands, intestinal fibrosis, acute to sub-acute suppurative inflammation with neutrophil infiltration and chronic inflammation with mononuclear cell infiltration. Serum alkaline phosphatase analysis did not show significant difference between mastitic and non mastitic cows. In conclusion, mastitis was one of diseases of dairy cows in the study areas which could affect dairy production. Some udder traits could be risk factors to mastitis and chronic mammary lesions characterized might severely decreased milk production and could be reason for culling of dairy cows. Therefore udder traits might be part of dairy cattle selection and improvement programs with detailed further study on the subject in the country. . Keywords: Alkaline phosphatase, Bacterial isolation, Cow, Ethiopia, Histopathology, Mastitis, Somatic cell count, and Udder morphometry

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Keywords

Alkaline phosphatase, Bacterial isolation, Cow, Ethiopia, Histopathology, Mastitis, Somatic cell count, and Udder morphometry

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