Comparative Study of Aflatoxin M1 in Milk Samples Marketed in Capital City and Rural Place
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Date
2017-01
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Addis Ababa University
Abstract
Milk is a key contributor to improve nutrition and food security particularly in developing
countries; moreover these products are widely consumed by children. Milk has the greatest
demonstrated potential for introducing AFM1 into the human diet and the possible presence of
AFM1 in milk is turn out to be critical concern of the country. Aflatoxin M1 (AFM1) is a major
carcinogenic compound that may be found in milk and dairy product resulting from ingestion of
aflatoxin B1 by dairy animals. This study compare the intensity of Aflatoxin M1 contamination
on milk from grazing and non- grazing cows collected from four potential milk source of the
country, Debrbrihan, Sululta and Bishoftu and Addis Ababa which is a major market. A total of
21 samples from grazing and non grazing cows were evaluated for their Aflatoxin (AFM1). The
main analytical technique implemented for aflatoxin analysis was Immunoaffinity column sample
clean-up and Shimadzu High Performance Liquid Chromatography (HPLC) using fluorescent as
a detector. Questionnaire also implemented to assess the knowledge, attitude and practice (KAP)
aspect of the participants. Results revealed that, Aflatoxin level of the milk samples from grazing
cows, in sululta Debereberhan and Bishoftu towns were in the range of (0.22-1.02) , (0-4.99)
and (0-7.57) respectively. The Aflatoxin level from the non-grazing cows ranged from (14.2-
28.96) Sululta, (3.28-42.87) Debereberhan and (3.21-43.47)in Bishoftu. The milk samples that
were collected from Addis Ababa city had ranged from aflatoxin concentration of (2.34-76.07).
One way anova results indicate that the mean value of cow’s that were grazing is significantly
different (P<0.05) from the milk samples of cow’s that were non-grazing and milk collected from
Addis Ababa.KAP results indicated negative relation between knowledge and aflatoxin
concentration whereas practice had positive relation. These results suggest that mitigation
should focus on type of feed and feed handling. Furthermore comprehensive and well-integrated
approach needs to follow the value chain actors to manage aflatoxin risks and to pull aflatoxins
out of human food chains.
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Key Words: milk,;Aflatoxin; grazing, non grazing; Immunoaffinity column;HPLC; value chain
Description
Keywords
milk,;Aflatoxin; grazing, non grazing; Immunoaffinity column;HPLC; value chain