Determine the Potency of Caffeine Degradation Microbes and Process Optimization
No Thumbnail Available
Date
2019-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababa University
Abstract
Bioremediation being a problem solver for many different environmental and other major
hazards, they play an important and cost effective role in the decaffeination process. Form
collected environmental sample coffee husk. From CH caffeine can be eliminated. With this
experimental work take two spp of microorganism that were bacillus subtilis and rhizobium from
Ethiopia bio diversity institute, that can be capability of improving soil fertility. Could grow on
the medium supplemented with 2.5g/L caffeine and could effectively degrade 2.5 g/L of caffeine
in the liquid media as a sole source of carbon and nitrogen. Morphological and biochemical
characteristics were maintained for re-affirmation the organism as bacillus subtilis and
rhizobium. The degradation of caffeine capability was authenticated by growth curve. The next
task were that those authenticated caffeine degrading bacteria applicable for conserving soil
fertility by means of N-fix and p- solubilized potential then propagation and growth of those
selective caffeine degrading bacteria was maintained by selective medium with and without
supplement of 2.5g/l caffeine then optimized the factors that affecting cell growth with prepared
broth with and without supplement of caffeine and determined interaction of each factor from
experiment design software 6.0.8) the result would be temp; 35
o
c, pH 7.07 incubation time 36hr
from those optimal condition obtained maximum cell growth in number was 3.41995E+008
and 5.0707E+008 with and without caffeine respectively for bacillus subtilis and temp 34
o
c, pH
6.6 and incubation time 28hrs optimum condition maximum cell growth 3.51146E+008 and
4.09131E+008 was obtained for rhizobium sp. of organism. That viable cell grown
microorganism ready for applicable of as liquid bio fertilizer those were easiest way of
production, handle, cost effective and keep soil health care method of production.
Description
Keywords
Coffee husk, caffeine, rhizobium, bacillus, subtilis, tannins, YEM, PIKOVSKAYA medium