Comparative study on the antibody response of infectious bursal disease immune-complex and live vaccines and survey on overall disease status progress and vaccine management in commercial poultry farms in Bishoftu Town, Central Ethiopia
| dc.contributor.advisor | Dr. Debebe Ashenafi | |
| dc.contributor.advisor | Dr. Bedaso Mammo | |
| dc.contributor.author | Nanoshe Taye | |
| dc.date.accessioned | 2024-11-04T11:34:31Z | |
| dc.date.available | 2024-11-04T11:34:31Z | |
| dc.date.issued | 2024 | |
| dc.description | MSc Thesis | |
| dc.description.abstract | The poultry production sector is vital for providing sustenance and enhancing food security in developing nations such as Ethiopia. However, this sector faces significant challenges, including infectious bursal disease (IBD). Evaluating the effectiveness of different vaccines for IBD is crucial for improving vaccination protocols and disease management. This study, conducted from November 2023 to May 2024, involved a controlled experimental study on 100 day-old chickens and a questionnaire survey of 93 poultry farms in Bishoftu town. The objectives were to gain insights into disease and vaccine management and to compare the immune response of chickens to various vaccines. The experimental study utilized a stratified randomized controlled trial with four groups of 25 chickens each. Group 1 received the CEVAC® TRANSMUNE vaccine, Group 2 received CEVAC® GUMBO L, Group 3 received IBD VIRUS LC – 75, and Group 4 served as a control. Vaccines were administered according to the manufacturer's recommendations. Blood samples (2 ml per chick) were collected on days 7, 14, 21, 28, 35, and 42 post-vaccination. Serum samples were analyzed for antibody response using a commercial indirect ELISA kit, and data were processed with STATA software. Survey results indicated that most respondents (64.52%) had 1 to 5 years of experience in the sector, and 50.54% were familiar with IBD and its impact. Key factors in vaccine selection included efficacy (45.16%), availability (19.35%), and cost (18.28%). Antibody titers produced by the vaccines varied: Trans-immune showed mean titers of 1298.814, 183.868, 38.864, 6280.42, 4424.76, and 6129.617 on days 7, 14, 21, 28, 35, and 42, respectively; Gumbo L showed titers of 21.46286, 2581.282, 3363.248, 3156.71, 3400.04, and 5973.526; and IBD LC-75 showed titers of 22.916, 642.136, 5270.4, 5653.071, 5793.221, and 6520.836 on the same days. All vaccines induced protective antibody titers, despite differences in the timing of responses. Continuous and updated surveillance on vaccine types and management practices is essential for effective disease control. Evaluating the immune response to vaccines in the presence of the challenge virus is also critical | |
| dc.identifier.uri | https://etd.aau.edu.et/handle/123456789/3533 | |
| dc.language.iso | en_US | |
| dc.publisher | Addis Ababa University | |
| dc.subject | Chickens | |
| dc.subject | Efficacy | |
| dc.subject | ELISA | |
| dc.subject | IBD | |
| dc.subject | Vaccine | |
| dc.title | Comparative study on the antibody response of infectious bursal disease immune-complex and live vaccines and survey on overall disease status progress and vaccine management in commercial poultry farms in Bishoftu Town, Central Ethiopia | |
| dc.type | Thesis |