Effects of chronic administration of aqueous Leaf Extracts Of T. Schimperi on Blood Parameters and histology of liver And kidney In Wistar Rats

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Addis Ababa Universty


Back ground: Since ancient time, plants have been used as indispensable sources of medicine and condiments. Nowadays, many traditional medicinal plants are incorporated in to modern medicine in many developed countries in the western world. However, in the developing countries like Ethiopia, traditional medicinal plants are still used in traditional manner without considering their effect, efficacy and dosage. T. schimperi is one of the traditional medicinal plants used for disease alleviation and food spice. This chronic study of toxicity was focused on evaluating the effects of chronic administration of aqueous leaf extracts of T. schympri on blood parameters and histology of liver and kidney in Wistar Rats. Methods and materials: Leaves of T. schimperi were collected from Bale Mountain, Oromia region and dried in the shade area at room temperature. The plant leaves then were powdered by pestle and mortar and macerated by aqueous water and shacked by orbital shaker. Finally, the macerated extract was filtered and freezed in deep freeze then dried in lyophilizer vacuum. For this study total of 40 rats, 20 male and 20 female rats, were used. Rats of each sex were randomly grouped into three experimental and one control group with 10 rats in each group 5 male and 5 females in separate cage. The experimental groups, II, III, and IV, were administered with the aqueous leaf extract at doses of 500, 1000, and 2000mg/kg, respectively for 180 days and then finally the rats were sacrificed humanly and liver and kidney and blood were taken for histopathology, hematology and biochemistry assessment. Results: The general behavior of the rats in treatment group was as normal as the control group. No statistical significant changes were seen between the mean bodyweight, liver and kidney weight and Blood parameters of experimental and control group of Wistar rats, however, considerable variations were observed between these parameters and mild focal mononuclear infiltration have been seen in the treatment of female rats treated with 1000and male rats treated with 2000mg/kg T. schimperi. Conclusion: Based on the findings of present chronic toxicity study it is difficult to draw conclusion with negative results because small sample size of animals was used rather it provides a baseline for future studies.



T. schimperi, chronic toxicity, medicinal plants and Wistar rats