Determination of Hematological Parameters Reference Interval for Adults of Dire Dawa Population, Dire-Dawa, Ethiopia

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Addis Ababa University


Background: Reference interval (RI) for hematological parameters are used for a comparative decision-making process by the treating physician, for diagnosis, management and monitoring of hematologic disorders. Manufacturers’ package inserts RI, which is western population based, is the most frequently external source of hematologic parameters. However, many factors like gender, age, environmental, lifestyle, and ethnicity influence hematological parameters. Objective: To establish hematological parameters reference interval for adults of Dire Dawa Population, Dire-Dawa. Methods: A cross sectional study design was employed for hematologic reference interval determination in Dire Dawa City Administration from January to May 2019. The study participants were from strata of adults (18-65 ages of both sexes) and pregnant women. After obtaining informed written consent, data was conveniently collected using a well-defined questionnaire that covers exclusion criteria. Fresh whole blood of 5ml was collected and 2ml poured into K3EDTA and analyzed by Mindray BC-3000plus hematology analyzer and 3ml for serology tests. Data was analyzed using SPSS version 24, the 95% reference intervals for each hematological parameters were calculated. Statistical significance was determined at p< 0.05. Result: A total of 472 individuals, aged 18 to 65 years for adult groups of both gender and 15 to 49 years for pregnant women were the final study participants in this study. Their mean ± SD age was 30.93±9.34 years. The respective 95% reference intervals for males, females and pregnant women were for WBC: 3.5-10.3x103/μl, 3.8-10.2x103/μl and 3.6-11.8 x103/μl; for Hgb: 12.40-17.50gm/dl, 10.78-15.25gm/dl and 9.5-13.50gm/dl; for RBC: 4.46-6.15x106/μl, 3.81-5.49 x106/μl and 3.67-5.07x106/μl; for HCT: 43.8-58.5%, 37.48-52.08% and 32.20-46.03%; for PLT: 164.0-447.00x103/μl, 177.0-442.27x103/μl and 157.5-421.5x103/μl. A statistically significant difference was observed between males and females for almost all hematologic parameters. Conclusion: There was a significant difference for most hematologic parameters based on genders and between pregnant and non-pregnant women. The RI obtained in this study is different from the values currently practiced in Dire Dawa town underscoring the need for locally appropriate intervals.



Reference Interval; Hematology; Adult men; Adult female; pregnant women