Analgesic and anti-inflammatory activities of Albizia gummifera (J. F. Gmel) C.A. Sm. bark hydro-alcoholic extract and its fractions in rodents
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Date
2023-10
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Addis Ababa University
Abstract
Background: The majority of illnesses present as pain and inflammation. Despite
advancement in pain medicines, there is a need for safe, effective analgesic drugs. In folkloric
medicine, A.gummifera has been used for pain and inflammation, but not scientifically
evaluated.
Objectives: This study aimed to investigate the analgesic and anti-inflammatory activities of
A.gummifera bark crude extract and its fractions in rodents.
Methods: The analgesic activity of the A.gummifera was evaluated using an acetic-acid-
induced writhing test and hot plate test by using acetyl salicylic acid 150 mg/kg and
morphine 10 mg/kg as standard, respectively. Carrageenan-induced paw edema and cotton
pellet granuloma methods were used to investigate the anti-inflammatory effect with
Indomethacin 10 mg/kg and dexamethasone 0.5 mg/kg as the reference drugs, respectively.
Three doses are selected based on the acute toxicity test result: 100 mg/kg, 200 mg/kg, and
400 mg/kg.
Results: The A.gummifera bark crude extract and its fractions did not show any sign of
toxicity at 2000 mg/kg. The crude extract and its fractions showed statistically significant
analgesic and anti-inflammatory activities as compared to the control group (p<0.05). The
crude extract and methanol fractions reduced writhes by 66.68% and elongate latency period
by 61.47% on 60 and 90 minute, respectively. The maximum edema inhibition was 62.15%
in the aqueous fraction after 2 hours of administration. Aqueous fraction produces maximum
exudate and granuloma inhibition (62.07% and 65.66%, respectively).
Conclusion: This study found that, A.gummifera bark crude extract and its fractions
possessed promising analgesic and anti-inflammatory property.
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Keywords
Pain, Analgesic, anti-inflammatory, Albizia gummifera.