In Vitro and in Vivo Antidiabetic Activity of 70 % Ethanolic Fruit Extract of Rosa Abyssinica R.Br. Ex Lindl (Rosaceae
No Thumbnail Available
Date
2024-02-10
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababa University
Abstract
Diabetes Mellitus (DM) is a metabolic disorder result from defects in insulin secretion, action or both. Given the high prevalence of disability and comorbidities associated with DM, it’s pivotal to bring forth sensible preventative and alternative treatment plans. Many plants in Rosacea family have been studied for their antidiabetic activity and Rosa abyssinica is one of the plant that widely used for its antidiabetic activity in traditional medicine in Ethiopia. The purpose of this study was to assess antihyperglycemic outcome of 70% ethanol extract of Rosa abyssinica. The in vitro antidiabetic effect was evaluated using assay for α-amylase inhibition of 70% ethanol fruit extract of R. abyssinica and positive standard, acarbose, at six different concentrations using 3,5-dinitrosalicylic acid (DNSA) technique. Conversely, normoglycemic, glucose-loaded, and streptozotocin (STZ)-induced diabetic mouse models were used to assess the in vivo antihyperglycemic activity. Five groups of mice—six mice in each group—were used in this investigation, consists of three experimental groups receiving 100, 200, and 400 mg/kg of the extract, positive control group receiving glibenclamide (GLC5 mg/kg), and negative control group receiving distilled water (10 ml/kg). In STZ-induced diabetes, a single intraperitoneal injection of 180 mg/kg body weight of STZ was used to cause diabetes. Anti-hyperglycemic effect of the extract in STZ -induced diabetic mice was evaluated using single dose and repeated dose study for three weeks. The in vitro test for α-amylase inhibition was analyzed using independent sample t test and the result showed there were no significant difference between the extract and the standard drug, acarbose, with triplicate measurement of IC50 of 26.72 ± 3.60 and 21.37 ± 4.25μg/ml respectively. The in vivo antihyperglycemic effect both in the oral glucose challenge and STZ induced diabetic mice showed similar result between the positive control and the two highest dose of the extract. In the normoglycemic experiment except with the highest dose of the extract, RA400, the other doses of the extract showed no hypoglycemic side effect and the extract showed positive for all tested qualitative secondary metabolite tests and quantitative test results showed alkaloids (83.37 mg ATP/g), phenols (892 mg GAE/g) and flavonoids (286.58 mg QCE/g) amounts.
Description
Keywords
IN VITRO AND IN VIVO ANTIDIABETIC