Green Synthesis of Honey based Silver Nanoparticles for Ampicillin Delivery against Drug Resistance Escherichia Coli
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Date
2023
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Addis Ababa University
Abstract
The efficiency of antibiotics is currently being limited by the critical public health issue of
antimicrobial resistance. Neonatal mengitus, which is predominantly caused by Escherichia coli (E.
coli), is the most common infection. Its management has becoming more challenging due to the
emergence of antibiotic resistance.
The goal of the current study was to synthesize AgNPs from honey's aqueous extract to deliver
ampicillin (AMP) and boost its potency against AMP-resistant E. coli. Important operating parameters
were under control. One factor at a time method was employed for optimizing the effects of
temperature, time, pH, silver nitrate and honey concentrations, and the ratio of the mixing amount of
aqueous extracted honey and silver nitrate solutions. AMP was loaded into AgNPs by changing the
incubation period and AMP concentration. The AMP-loaded AgNPs were characterized using UV-vis
spectroscopy, Fourier transform infrared (FTIR), X-ray diffraction (XRD), differential scanning
calorimetry (DSC), Dynamic light scattering (DLS), and Scanning electron microscope (SEM). The in
vitro drug release profile AMP loaded AgNPs was evaluated at pH 1.2, 6, 7.3, 7.4 and 8. The in vitro
antibacterial capability of AMP, AgNPs and AMP loaded AgNPs and honey against E.coli was
examined using the disc diffusion method.
The optimized AgNPs had a 49 0.572nm crystal size and a spherical and uneven shape. X-ray
energy dispersive spectroscopy, verified the purity of the manufactured AgNPs. UV-visible and FTIR
spectra of the AMP loaded AgNPs revealed separate bands for both the AMP and AMP loaded
AgNPs. Optimized AMP-loaded AgNPs had loading contents of 48.08 % and 96.3% entrapment
efficiency. A regulated release of AMP over 24 hours was achieved with a pH medium of 7.3 and pH
7.4. Inhibitory zones for AgNPs, and AMP loaded AgNPs were 10.00± 0.6673 and 17.2 1.686mm,
respectively where it was null for honey and AMP. AMP loaded AgNPs demonstrated substantially
potent antibacterial action as compared to AMP and AgNPs alone. Thus, against AMP-resistant E.
coli, the AMP-loaded AgNPs has shown encouraging results.
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Keywords
Ampicillin, Ampicillin loaded silver nanoparticle, Antimicrobial resistance, Green synthesis, Honey extract, Silver nanoparticles