Browsing by Author "Yibrah Tekle"

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    Isolation and Molecular Characterization of Mycobacterium Bovis Isolated from Cattle Slaughtered at Hawassa University and Municipal Abattoirs, Southern Ethiopia
    (Addis Ababauniversity, 2016-06) Yibrah Tekle ; Mamo Gezahegn
    A cross sectional study was conducted on 753 Cattle, selected using systematic random sampling technique, in Hawassa abattoirs, Southern Ethiopia from December 2015 to May 2016 to investigate the prevalence of bovine tuberculosis (BTB), identifying the risk factors as well as to isolate and characterize its causative agent. The methods used were postmortem examination, bacteriological culturing, RD4 deletion typing and Spoligotyping. The overall prevalence of the bovine tuberculosis was 5.8% (95%CI: 4.16-7.52) on the basis of detailed postmortem examination. Multivariable logistic regression analysis identified age, body condition; breed and market were statistically significant. The older cattle above 8 years were eighteen times (OR=18.25) more likely to have tuberculosis than the younger cattle. The cross breeds were nine times (OR = 9.75) more sensitive to M. bovis as compared to local breeds as well as the poor body conditioned cattle forty one times (OR = 41.21) more likely to have tuberculosis than the cattle have good body condition. Relatively, the occurrence of BTB was higher in cattle brought from Hawassa (OR = 13.17) and Borena (OR = 9.41) markets than Tula market. The lesions were found most frequently distributed in thoracic cavity lymph nodes (75%), which indicated that respiratory route was the main mode of infection in the study area. Out of 44 tissue samples cultured, 24 (54.5%) were culture positive and all were acid fast staining positive. However, using RD4 deletion only three isolates were confirmed as M. bovis.The further Spoligotyping characterizations of these three isolates reveal ed the strain SB1477. And the rest isolates that have never showed any signal (21/24) in the RD4 deletion typing should be characterized by using the mPCR in order to confirm either they are Mycobacterium tuberculosis complex or notuberculosis mycobacterium. In conclusion, the study confirmed that the low prevalence of BTB and the current M. bovis strain circulate in the cattle being SB1477 that indicate implement contr ol program and investigate the zoonotic role of this strain. Key words: Culturing, Hawassa abattoirs, M. bovis, Postmortem, RD4 deletion, Spoligot yping, ZN Staining