Isolation and Molecular Characterization of Mycobacterium Bovis Isolated From Cattle Slaughtered at Hawassa University and Municipal Abattoirs, Southern Ethiopia
No Thumbnail Available
Date
2016-06
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Addis Ababauniversity
Abstract
A cross sectional study was conducted on 753 Cattle, selected using systematic random
sampling technique, in Hawassa abattoirs, Southern Ethiopia from December 2015 to
May 2016 to investigate the prevalence of bovine tuberculosis (BTB), identifying the risk
factors as well as to isolate and characterize its causative agent. The methods used were
postmortem examination, bacteriological culturing, RD4 deletion typing and
Spoligotyping. The overall prevalence of the bovine tuberculosis was 5.8% (95%CI:
4.16-7.52) on the basis of detailed postmortem examination. Multivariable logistic
regression analysis identified age, body condition; breed and market were statistically
significant. The older cattle above 8 years were eighteen times (OR=18.25) more likely to
have tuberculosis than the younger cattle. The cross breeds were nine times (OR = 9.75)
more sensitive to M. bovis as compared to local breeds as well as the poor body
conditioned cattle forty one times (OR = 41.21) more likely to have tuberculosis than the
cattle have good body condition. Relatively, the occurrence of BTB was higher in cattle
brought from Hawassa (OR = 13.17) and Borena (OR = 9.41) markets than Tula market.
The lesions were found most frequently distributed in thoracic cavity lymph nodes
(75%), which indicated that respiratory route was the main mode of infection in the study
area. Out of 44 tissue samples cultured, 24 (54.5%) were culture positive and all were
acid fast staining positive. However, using RD4 deletion only three isolates were confirmed
as M. bovis.The further Spoligotyping characterizations of these three isolates reveal
ed the strain SB1477. And the rest isolates that have never showed any signal (21/24) in
the RD4 deletion typing should be characterized by using the mPCR in order
to confirm either they are Mycobacterium tuberculosis complex or notuberculosis mycobacterium.
In conclusion, the study confirmed that the low prevalence of BTB and the
current M. bovis strain circulate in the cattle being SB1477 that indicate implement contr
ol program and investigate the zoonotic role of this strain.
Key words: Culturing, Hawassa abattoirs, M. bovis, Postmortem, RD4 deletion, Spoligot
yping, ZN Staining
Description
Keywords
Culturing, Hawassa abattoirs, M. bovis, Postmortem, RD4 deletion, Spoligotyping, ZN Staining