Browsing by Author "Tibebu, Melatwork(MSc, PhD candidate)"

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    Determination of Hematological Parameters Reference Interval for Adults of Dire Dawa Population, Dire-Dawa, Ethiopia
    (Addis Ababa University, 2019-06) Mengistu, Teklay; Tsegaye, Aster(MSc, PhD); Tibebu, Melatwork(MSc, PhD candidate)
    Background: Reference interval (RI) for hematological parameters are used for a comparative decision-making process by the treating physician, for diagnosis, management and monitoring of hematologic disorders. Manufacturers’ package inserts RI, which is western population based, is the most frequently external source of hematologic parameters. However, many factors like gender, age, environmental, lifestyle, and ethnicity influence hematological parameters. Objective: To establish hematological parameters reference interval for adults of Dire Dawa Population, Dire-Dawa. Methods: A cross sectional study design was employed for hematologic reference interval determination in Dire Dawa City Administration from January to May 2019. The study participants were from strata of adults (18-65 ages of both sexes) and pregnant women. After obtaining informed written consent, data was conveniently collected using a well-defined questionnaire that covers exclusion criteria. Fresh whole blood of 5ml was collected and 2ml poured into K3EDTA and analyzed by Mindray BC-3000plus hematology analyzer and 3ml for serology tests. Data was analyzed using SPSS version 24, the 95% reference intervals for each hematological parameters were calculated. Statistical significance was determined at p< 0.05. Result: A total of 472 individuals, aged 18 to 65 years for adult groups of both gender and 15 to 49 years for pregnant women were the final study participants in this study. Their mean ± SD age was 30.93±9.34 years. The respective 95% reference intervals for males, females and pregnant women were for WBC: 3.5-10.3x103/μl, 3.8-10.2x103/μl and 3.6-11.8 x103/μl; for Hgb: 12.40-17.50gm/dl, 10.78-15.25gm/dl and 9.5-13.50gm/dl; for RBC: 4.46-6.15x106/μl, 3.81-5.49 x106/μl and 3.67-5.07x106/μl; for HCT: 43.8-58.5%, 37.48-52.08% and 32.20-46.03%; for PLT: 164.0-447.00x103/μl, 177.0-442.27x103/μl and 157.5-421.5x103/μl. A statistically significant difference was observed between males and females for almost all hematologic parameters. Conclusion: There was a significant difference for most hematologic parameters based on genders and between pregnant and non-pregnant women. The RI obtained in this study is different from the values currently practiced in Dire Dawa town underscoring the need for locally appropriate intervals.
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    The diagnostic predictive value of platelet count and platelet indices in patients with acute coronary syndrome: A comparative cross sectional study at TikurAnebesa Specialized Hospital, Addis Ababa, Ethiopia.
    (Addis Ababa University, 2023-09) Gera, Kalkidan; Urgessa, Fekadu(MSc, PhD candidate); Tibebu, Melatwork(MSc, PhD candidate)
    Background:Acute coronary syndrome(ACS) is the leading cause of death worldwide . The abnormality of Platelet and platelet indices are one of the most commonly identified hematological changes in ACS. Objective:To determine the diagnostic predictive value of platelet count and platelet indices in patients with ACS at TikurAnbesa Specialized Hospital, Addis Ababa, Ethiopia from April 2021-March2023. Methods:Hospital based comparative cross-sectional study was conducted among 76 ACS patients of cases and 152 controls (apparently healthy individuals) in TikurAnbesa specialized hospital(TASH)in Addis Ababa conducted from April 2021 to March 2023. Convenient sampling technique was used. Independent t testwas used for comparison betweenthe twogroups.ROC(receiver operating characteristic) curve test used to calculate positive and negative predictive values, sensitivity and specificity for a given platelet indices. Data was entered and analyzed using SPSS version 25 and level of significance was determined when P value was < 0.05. Result:The mean age of ACS group(50.91±11.33) and control group (51.85±11.7) was matched. Males study participants predominated females in all groups.The mean of Mean Platelet Volume(MPV) (11.66±.1.19)fl, Platelet Large Cell Ratio(PLCR) (37.35 ± 9.58)% and Platelet Distribution Width(PDW) (15.10 ±3.53)fl of ACS group was significantly higher than the control group (10.17±1.24)fl, (26.55 ±11.20)%,(11.52 ±2.88)fl, respectively with (p value <0.001) . In Roc analysis MPV ,PDW, PLCR had cut off value of >10.9fl, >12.7fl, >33.2%, value, respectively and can differentiate ACS from healthy individuals with Sensitivity of 84.2%,78.9%76.3%, Specificity of 75%,67.1%74.3%,Postive Predictive value(PPV)of 66%,54.1%,61.1% and Negative predictive value (NPV) of 90.4%,86.3%,86.3% ,respectively. The Area under Curve (AUC) of MPV, PDW and PLCR was 0.788, 0.767 and 0.746. Conclusion: The mean ofMPV, PDW and PLCR were significantly increased in ACS compared to control group value .MPV has the highest value of sensitivity, specificity, NPV and PPV followed by PLCR then PDW. These parameters could be used as a supportive tool for prediction of ACS disease on the top of the clinical diagnosis and advanced tests set up.