Browsing by Author "Tazeb Adebabay"

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    Molecular Characterization of Introduced Sugarcane (Saccharum officinarum L.) Genotypes in Ethiopia Using Inter Simple Sequence Repeat (ISSR) Markers
    (Addis Ababa University, 2015-03) Tazeb Adebabay; Haileselassie Teklehaimanot ; Tesfaye Kassahun
    The major thrust of sugarcane (Saccharum officinarum L.) variety improvementprograms is to increase sugar yield, while due to its genetic complexity, sugarcane hasreceived very little research interest, despite its economic importance, and moleculartechniques are being developed only in recent times. Furthermore, until the present dateno molecular studies were carried out to evaluate sugarcane germplasm from Ethiopianfields. Therefore, the objective of this study was to evaluate genetic diversity andestablish relationships among populations of sugarcane in Ethiopia using ISSR markers.Genomic DNA was extracted from leave samples of 82 sugarcane genotypes according tomodified CTAB method. A total of 149 scorable and reproducible bands were generatedusing 12 ISSR primers among which 124 were polymorphic and attributed to PPL =83.22 %, h = 0.31, and I = 0.45 at species level. Intra-population diversity based onpolymorphic loci ranged from 28.86 % to 47.65 % with mean of 38.35 %, Nei’s genediversity of 0.097 to 0.171 with mean of 0.137, Shannon’s information index of 0.147 to0.255 with mean of 0.205, and within population AMOVA of 63.56 %. All diversityparameters confirm that, the highest diversity was obtained from those that were obtainedfrom France and Cuba whilst the lowest was from those of Barbados and South Africa.AMOVA revealed 36.4 % among population variation which is significantly lower thanthat of within population variation. From Jaccard’s pairwise similarity coefficient, thosefrom Sudan and South Africa were most related populations exhibiting 0.70 similarityand USA and Barbados were the most distantly related populations with similarity of0.53. With all clustering analysis, most of the genotypes clustered to their respectiveorigins. Thus, ISSR markers detected a range of diversity from sugarcane varieties withtheir unique identity that deserve conservation attention and improvement programs. Key words: Commercial crop, genetic diversity, ISSR markers, Saccharum officinarum