Browsing by Author "Taticheff, Seyoum"

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    The Application of the Enzyme Linked immunosorbent Assay (Elisa) In t'he Sehodiagnosis of Schistosoniasis
    (Addis Ababa University, 1980-06) Taticheff, Seyoum; Melamed, M.D. (PhD)
    A description of the schistosome parasite and its life cycle, its immunology and the fact that the parasite causes a disease of world-wide significance is made. The existing distribution of schistosomiasis in Ethiopia and its recorded vectors and the possibility that the disease may rapidly be coming a public health threat is mentioned. As possible tools for the sero-diagnosis of schistosomiasis, short accounts of the skin test, the indirect immuno-fluorescence test, the complement fixation test and the radio-immunoassay are gi ven. '['he enzyme linked immunosorben t assay (ELISA) as a promising diagnostic method has been elaborated '['he primary objective of this study was the assessement of ELISA as a sero-epidemiologic tool for schistosomiasis. In this study, samples from S .mansoni and- S .haematob.iill!! endemic areas and samples from non-endemic area were used. ~ljicroscopic examination of stool and/or urine ELISA on blood samples and skin tests for schistosomiasis were done. ELISA with a sensitivity of 86.86 to 96% and a specificity or 80 to 81% was preferred as a sero-diagnostic method for schistosomiasis from: the conventional microscopy where sensitivity was 101'1 and from the intradermal test which showed poor specifici ty (40% posi ti vi ty was observed in" non-endemi c area).