The Application of the Enzyme Linked immunosorbent Assay (Elisa) In t'he Sehodiagnosis of Schistosoniasis
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Date
1980-06
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Addis Ababa University
Abstract
A description of the schistosome parasite and its life cycle,
its immunology and the fact that the parasite causes a disease of
world-wide significance is made. The existing distribution of
schistosomiasis in Ethiopia and its recorded vectors and the
possibility that the disease may rapidly be coming a public health
threat is mentioned. As possible tools for the sero-diagnosis of schistosomiasis,
short accounts of the skin test, the indirect immuno-fluorescence
test, the complement fixation test and the radio-immunoassay are
gi ven. '['he enzyme linked immunosorben t assay (ELISA) as a promising
diagnostic method has been elaborated '['he primary objective of this study was the assessement of
ELISA as a sero-epidemiologic tool for schistosomiasis. In this
study, samples from S .mansoni and- S .haematob.iill!! endemic areas and
samples from non-endemic area were used. ~ljicroscopic examination
of stool and/or urine ELISA on blood samples and skin tests for
schistosomiasis were done. ELISA with a sensitivity of 86.86 to
96% and a specificity or 80 to 81% was preferred as a sero-diagnostic
method for schistosomiasis from: the conventional microscopy
where sensitivity was 101'1 and from the intradermal test which showed
poor specifici ty (40% posi ti vi ty was observed in" non-endemi c area).
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Biology